8,060 results

1. pGBW-m4046489

   Plasmid

   #145554

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBW-m4046335

   Plasmid

   #145534

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046346

   Plasmid

   #145390

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pAG425GPD-ccdB-EYFP

   Plasmid

   #14226

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG305GPD-EGFP-ccdB

   Plasmid

   #14306

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. piHGpd/G170D

   Plasmid

   #1113

   Depositor

   Susan Lindquist

   Article

   Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

   Insert

   HSP82 (HSP82 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   HSP82 with glycine 170 changed to aspartic acid; …

   Promoter

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pKT0221

   Plasmid

   #8726

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yECFP
   

   Use

   Tags

   3HA and Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

   Promoter

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG425GAL-ccdB-EYFP

   Plasmid

   #14225

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PP165

   Plasmid

   #78985

   Purpose

   Supplemental Figure 4. Promoter GCW14. Expresses GFP.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ZJOM8

    Plasmid

    #133671

    Purpose

    Integration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SNF7-GFP (SNF7 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

    Plasmid

    #58830

    Purpose

    Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

    Insert

    2 x Osh2 PH domain (OSH2 Budding Yeast)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    changed Lysine 307 to Glutamate and Arginine 309 …

    Promoter

    PHO5

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

12. LD223

    Plasmid

    #69190

    Purpose

    Integration of 2GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg23-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ube2d3

    Plasmid

    #31411

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2D3 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pYZ159

    Plasmid

    #98415

    Purpose

    Pombe Expression Vector - nmt41 promoter with Sp.leu1 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG425GAL-EYFP-ccdB

    Plasmid

    #14345

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNX3a-FL3

    Plasmid

    #78358

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xFLAG
    kanMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG414GPD-EYFP-ccdB

    Plasmid

    #14336

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNC1053

    Plasmid

    #20406

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    linker: delta k

    Promoter

    Available Since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCuGFP-SpAtg17(416)

    Plasmid

    #122080

    Purpose

    Expressing S. pombe GFP-Atg17

    Depositor

    Daniel Klionsky

    Article

    Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

    Insert

    SpATG17 (atg17 Fission Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWS939

    Plasmid

    #123051

    Purpose

    Pheromone responsive transcription factor

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Ste12 [3]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge