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pDS1209

Plasmid

#177340

Purpose

Expresses Aequorin in Candida albicans using the URA3 auxotrophic marker URA3

Depositor

Dominique Sanglard

Publication

Sanglard et al J Fungi (Basel). 2021 Apr 20;7(4). pii:

Insert

Aequorin

Expression

Yeast

Promoter

ACT1

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-msGFP2-STE13

Plasmid

#177106

Purpose

Expresses msGFP2-Ste13 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

STE13 (STE13 Budding Yeast)

Expression

Yeast

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-iGFP

Plasmid

#177108

Purpose

Expresses Nhx1-iGFP in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Expression

Yeast

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-ANP1-msGFP2

Plasmid

#177100

Purpose

Expresses Anp1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ANP1 (ANP1 Budding Yeast)

Expression

Yeast

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-STV1-iGFP

Plasmid

#177111

Purpose

Expresses Stv1-iGFP in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

STV1 (STV1 Budding Yeast)

Expression

Yeast

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_2_dCas9_VP64_sgRNA

Plasmid

#163707

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

Depositor

Yvonne Nygård

Publication

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VP64+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL669

Plasmid

#169148

Purpose

Encodes OptoQ-AMP2

Depositor

Jose Avalos

Publication

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL722

Plasmid

#169149

Purpose

Encodes OptoQ-AMP3

Depositor

Jose Avalos

Publication

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL716

Plasmid

#169150

Purpose

Encodes OptoQ-AMP4

Depositor

Jose Avalos

Publication

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-HIS3

Plasmid

#122887

Purpose

LYS2-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-MET17

Plasmid

#122889

Purpose

LYS2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-LEU2

Plasmid

#122892

Purpose

TRP1-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-LEU2

Plasmid

#122884

Purpose

ADE2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

Plasmid

#173462

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDB1751

Plasmid

#174013

Purpose

pDUAL plasmid expressing GFP from 41nmt1 promoter

Depositor

Li-Lin Du

Publication

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

GFP

Tags

GFP

Expression

Yeast

Promoter

41nmt1 and 41nmt1 promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJKW1847

Plasmid

#174004

Purpose

Expresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Publication

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP94D108
ATR1 (ATR1 Mustard Weed)

Expression

Yeast

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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U6-U57C

Plasmid

#169928

Purpose

WT U6 with a U57C mutation

Depositor

Aaron Hoskins

Publication

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57C

Expression

Bacterial and Yeast

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGBK CUP1 mCherry

Plasmid

#170264

Purpose

Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mCherry

Depositor

Bruce Hamilton

Publication

Hernandez et al bioRxiv

Insert

momeric Cherry

Tags

GAL4 DNA Binding Domain and Myc

Expression

Bacterial and Yeast

Promoter

CUP1

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGBK CUP1 Empty

Plasmid

#169710

Purpose

Yeast two-hybrid vector using a copper inducible CUP1 promoter

Depositor

Bruce Hamilton

Publication

Hernandez et al bioRxiv

Type

Empty backbone

Tags

GAL4 DNA Binding Domain and Myc

Expression

Bacterial and Yeast

Promoter

CUP1

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Publication

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Chicken, Synthetic)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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