A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12.
Camara E, Lenitz I, Nygard Y
Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w. PubMed Article
Camara E, Lenitz I, Nygard Y
Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163707 | EC2_2_dCas9_VP64_sgRNA | Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain |
| 163708 | EC2_3_dCas9_Mxi1_sgRNA | Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor |
| 163709 | EC2_4_dCas9_VPR_sgRNA | Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains |
| 163710 | EC2_1_dCas9_sgRNA | Yeast low copy plasmid with dCas9 and sgRNA expression cassette |
| 163711 | EC2_7_dCas9_HC_sgRNA | High copy plasmid with dCas9 and sgRNA expression cassettes |
| 163712 | EC2_10_dCas9_VPR_HC_sgRNA | Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains |
| 163714 | EC0_2_VP64 | Plasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit) |
| 163715 | EC0_3_Mxi1 | Plasmid encoding Mxi1 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit) |
| 163716 | EC0_4_VPR | Plasmid encoding VPR (VP64 - SV40 NLS - p65 - Rta) as a Type 4a part to be used in the Dueber YTK system (MoClo kit) |
| 163717 | EC0_6_sgRNA | Plasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit) |
| 184757 | pYN2_1 | Plasmid for genome editing by CRISPR/Cas9 |