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pBCSK_LEU2_I

Plasmid

#109060

Purpose

yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposes

Depositor

Colin Logie

Insert

LEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)

Use

Cre/Lox

Expression

Bacterial and Yeast

Promoter

LEU2

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCTD26-S>A10-17

Plasmid

#112826

Purpose

pSMF2 with all S to A mutations in only repeats 10-17.

Depositor

Stephen Fuchs

Publication

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 1…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCTD26-S>A2-9

Plasmid

#112825

Purpose

pSMF2 with all S to A mutations in only repeats 2-9.

Depositor

Stephen Fuchs

Publication

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 2…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCTD26-S>A18-25

Plasmid

#112827

Purpose

pSMF2 with all S to A mutations in only repeats 18-25.

Depositor

Stephen Fuchs

Publication

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 1…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD8

Plasmid

#112820

Purpose

pSMF2 with 8 CTD repeats

Depositor

Stephen Fuchs

Publication

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRS303 prp8-101

Plasmid

#111307

Purpose

prp8-101

Depositor

Christine Guthrie

Publication

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

prp8-101 (E1960K)

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD10

Plasmid

#112821

Purpose

pSMF2 with 10 CTD repeats

Depositor

Stephen Fuchs

Publication

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314-SNU114

Plasmid

#111309

Purpose

pRS314-SNU114

Depositor

Christine Guthrie

Publication

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Expression

Yeast

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD2-ODPAMTS (12)

Plasmid

#101694

Purpose

ODPAMTS in domestication vector

Depositor

Elena Caro Bernat

Publication

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae Pyruvate dehydrogenase E1 component subunit alpha (YER178W)]-AATG

Expression

Yeast

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUPD2-ODPBMTS (12)

Plasmid

#101695

Purpose

ODPBMTS in domestication vector

Depositor

Elena Caro Bernat

Publication

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae Pyruvate dehydrogenase E1 component subunit beta (YBR221C)]-AATG

Expression

Yeast

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVEG17*

Plasmid

#111856

Purpose

Xylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)

Depositor

Nikhil Nair

Publication

Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

Insert

GAL1p-GAL3-Syn4.1-TEFt

Use

Synthetic Biology

Expression

Yeast

Mutation

D68N, V69M, A109V, I271L

Promoter

GAL1p

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB6581

Plasmid

#106137

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS316Nab3

Plasmid

#110533

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Publication

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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prp8-G736E

Plasmid

#108450

Purpose

prp8-G736E in pRS313 backbone for expression in yeast

Depositor

Christine Guthrie

Publication

Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

changed G736 to E

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS16-iGFPx6

Plasmid

#105275

Purpose

Plasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps16 portion (VPS16 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pKP115 (pRS406-YBR139Wp-YBR139W-GFP-ADH1t)

Plasmid

#106465

Purpose

Expresses Atg42/Ybr139w with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Publication

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W

Tags

GFP

Expression

Bacterial and Yeast

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t)

Plasmid

#106471

Purpose

Expresses Prc1 with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Publication

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Tags

PA

Expression

Bacterial and Yeast

Promoter

PRC1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBW105

Plasmid

#111137

Purpose

For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

Depositor

Alexander Varshavsky

Publication

Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

Insert

MLS-ratAANAT-3FLAG (Aanat Rat)

Tags

3x Flag

Expression

Yeast

Promoter

Gal1

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRS DV DNM2-mRUBY2

Plasmid

#107793

Purpose

donor vector for knock-in gene editing at human DNM2 locus

Depositor

Sandra Schmid

Publication

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

DNM2-mRuby2 (DNM2 Human, Synthetic)

Use

Yeast-e.coli shuttle vector

Expression

Bacterial and Yeast

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRS DV DNM1-EGFP

Plasmid

#107794

Purpose

donor vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Publication

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

DNM1-eGFP (DNM1 Human, Synthetic)

Use

Yeast-e.coli shuttle vector

Expression

Bacterial and Yeast

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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