8,194 results
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Plasmid#228287Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-EWSR1
Plasmid#181725PurposeGalactose inducible expression of EWSR1DepositorInsertEWSR1 (EWSR1 Human)
ExpressionYeastAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-hnRNPA2B1
Plasmid#181714PurposeGalactose inducible expression of hnRNPA2B1DepositorInserthnRNPA2B1 (HNRNPA2B1 Human)
ExpressionYeastAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-CFAPorig
Plasmid#221092PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneExpressionYeastAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3
Plasmid#221112PurposeFAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3
ExpressionYeastPromoterSTE3Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-STE3-GFP
Plasmid#221113PurposeGFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)DepositorInsertSTE3-GFP
ExpressionYeastPromoterTEF1Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 E3GFP
Plasmid#233008PurposeGalactose iduced expression of Gcn4 E+GFP in yeastDepositorInsertGcn4 E+
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationD105E, D118E, D139EPromoterGal1Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del1-51
Plasmid#234136PurposeFirst 51 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
ExpressionYeastMutationN-terminus truncationAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 S1584F
Plasmid#234122PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
ExpressionYeastMutationS1584FAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 817 Truncation
Plasmid#234126PurposeSyf1 PCR truncation starting at 817 through the end of the geneDepositorInsertSyf1
ExpressionYeastMutationN-terminus truncationAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 S1584Y
Plasmid#234121PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
ExpressionYeastMutationS1584YAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 V1862L
Plasmid#234123PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
ExpressionYeastMutationV1862LAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124IHA
Plasmid#232993PurposeGalactose iduced expression of Gcn4 F124IHA in yeastDepositorInsertGcn4 F124I
Tags3xHA and TEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_FL Gcn4
Plasmid#232955PurposeGalactose iduced expression of full-length Gcn4 in yeastDepositorInsertGcn4
TagsTEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del1-16
Plasmid#234138PurposeFirst 16 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
ExpressionYeastMutationN-terminus truncationAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del103-173
Plasmid#234134PurposeAmino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
ExpressionYeastMutationC-terminus truncationAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Fyv6-CFLAG
Plasmid#234133PurposeRestriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
ExpressionYeastAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del134-173
Plasmid#234135PurposeAmino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
ExpressionYeastMutationC-terminus truncationAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Fyv6
Plasmid#234140PurposeAmplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414DepositorInsertFyv6
ExpressionYeastAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-NFLAG-Fyv6
Plasmid#234139PurposeRestriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
ExpressionYeastAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only