8,103 results

1. pYAMTr2GCsgSc-SeLEU2

   Plasmid

   #224871

   Purpose

   Disruption of LEU2 genes in the Saccharomyces sense strict group species

   Depositor

   Katsunori Suzuki

   Article

   Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

   Insert

   pYAMTr2GC having the guide sequence Sc-SeLEU2 (LEU2, DI49_0736 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBSsgRNA

   Plasmid

   #224867

   Purpose

   Yeast genome editing, Insertion of 20 bp oligo into sgRNA gene

   Depositor

   Katsunori Suzuki

   Article

   Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNK7185

   Plasmid

   #219765

   Purpose

   Vector for expression PzPKS2 in yeast (P.pastoris)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   PzPKS2
   

   Expression

   Yeast

   Promoter

   pGAP

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYAMTr2GCsgScLEU2

   Plasmid

   #224868

   Purpose

   Disruption of S. cerevisiae type LEU2 gene

   Depositor

   Katsunori Suzuki

   Article

   Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

   Insert

   pYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS416 CUP1pro-Htt103QdeltaP125-134-YFP

   Plasmid

   #224882

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP125-134 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP125-134

   Promoter

   CUP1

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS416 CUP1pro-Htt103QdeltaP135-170-YFP

   Plasmid

   #224878

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP135-170 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP135-170

   Promoter

   CUP1

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS416 CUP1pro-Htt103QdeltaP141-160-YFP

   Plasmid

   #224886

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP141-160 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP141-160

   Promoter

   CUP1

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS415 GPDpro-RNQ1-CFP

   Plasmid

   #224887

   Purpose

   Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).

   Depositor

   Paul Herman

   Insert

   RNQ1 (RNQ1 Budding Yeast)
   

   Tags

   CFP

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS416 CUP1pro-Htt103QdeltaP125-160-YFP

   Plasmid

   #224883

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP125-160 (HTT Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP125-160

   Promoter

   CUP1

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS416 CUP1pro-M-PRR-Htt97QP4-YFP

    Plasmid

    #224885

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.

    Depositor

    Paul Herman

    Insert

    M-PRR-Htt97QP4 (HTT Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    N-PRR

    Promoter

    CUP1

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS416 CUP1pro-Htt103QdeltaP141-170-YFP

    Plasmid

    #224880

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.

    Depositor

    Paul Herman

    Insert

    Htt103QdeltaP141-170 (HTT Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    deltaP141-170

    Promoter

    CUP1

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS416 CUP1pro-Htt103QdeltaP161-170-YFP

    Plasmid

    #224876

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.

    Depositor

    Paul Herman

    Insert

    HTT103QdeltaP161-170 (HTT Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    delta161-170

    Promoter

    CUP1

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJJB1426

    Plasmid

    #218622

    Purpose

    Express tNCS to the peroxisome for toxicity assay

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    tNCS
    

    Tags

    YFP-ePTS1

    Expression

    Yeast

    Mutation

    106-588

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJJB1462

    Plasmid

    #218623

    Purpose

    Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    YFP
    

    Tags

    ePTS1

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJJB1463

    Plasmid

    #218624

    Purpose

    Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    YFP
    

    Tags

    ePTS1

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJJB1465

    Plasmid

    #218625

    Purpose

    Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Adr1
    

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJJB1734

    Plasmid

    #218627

    Purpose

    Express amorphadiene synthase (ADS)

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    ADS
    

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJJB1503

    Plasmid

    #218610

    Purpose

    Express Pex30

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex30
    

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJJB1504

    Plasmid

    #218611

    Purpose

    Express Pex31

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex31
    

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJJB1505

    Plasmid

    #218612

    Purpose

    Express Pex35

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex35
    

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge