7,943 results

1. pITEu2

   Plasmid

   #218282

   Purpose

   Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJE13HD7

   Plasmid

   #218281

   Purpose

   Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pILGFP4QE2Crimson

   Plasmid

   #218280

   Purpose

   Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIRHDGAL43C

   Plasmid

   #218279

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIRGAL43C

   Plasmid

   #218278

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P)

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIRGAL4A

   Plasmid

   #218277

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIRHDGAL3C

   Plasmid

   #218276

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIRGAL3C

   Plasmid

   #218275

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P)

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPOM014_P2_pGPM1

   Plasmid

   #216439

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter gpm1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRH3128

    Plasmid

    #210165

    Purpose

    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1

    Depositor

    Randy Hampton

    Article

    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

    Insert

    YIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1 (ADE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRH2941

    Plasmid

    #210143

    Purpose

    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1

    Depositor

    Randy Hampton

    Article

    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

    Insert

    YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1 (ARO7 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG426-GAL-Prepro-3x-Beta-Amyloid

    Plasmid

    #181730

    Purpose

    Galactose inducible expression of Prepro-3x-Beta-Amyloid

    Depositor

    Alejandro Chavez

    Insert

    Prepro-3x-Beta-Amyloid
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG426-GAL-Prepro-3x-Adan

    Plasmid

    #181733

    Purpose

    Galactose inducible expression of Prepro-3x-Adan

    Depositor

    Alejandro Chavez

    Insert

    Prepro-3x-Adan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG416-GAL-TDP-43-M337V

    Plasmid

    #181721

    Purpose

    Galactose inducible expression of TDP-43-M337V

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    M337V

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG416-GAL-TDP-43-N267S

    Plasmid

    #181720

    Purpose

    Galactose inducible expression of TDP-43-N267S

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N267S

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG416-GAL-TDP-43-Q331K

    Plasmid

    #181719

    Purpose

    Galactose inducible expression of TDP-43-Q331K

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q331K

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG416-GAL-FUS-R521C

    Plasmid

    #181718

    Purpose

    Galactose inducible expression of FUS-R521C

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R521C

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG416-GAL-FUS-P431L

    Plasmid

    #181717

    Purpose

    Galactose inducible expression of FUS-P431L

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    P431L

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG426-GAL-Kar2-Beta-Amyloid

    Plasmid

    #181707

    Purpose

    Galactose inducible expression of Kar2-Beta-Amyloid

    Depositor

    Alejandro Chavez

    Insert

    Kar2-Beta-Amyloid
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG416-GAL-RNQ1

    Plasmid

    #181706

    Purpose

    Galactose inducible expression of RNQ1

    Depositor

    Alejandro Chavez

    Insert

    RNQ1 (RNQ1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge