8,060 results

1. pEM.P01L

   Plasmid

   #198641

   Purpose

   Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding ends

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Insert

   pTDH3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Aga2p-LOV-Turbo1-myc_pCTCON2

   Plasmid

   #199673

   Purpose

   expresses pre-evolved version of LOV-Turbo on the yeast surface

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo1
   

   Use

   Tags

   Aga2p and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGBT9-FHIP

   Plasmid

   #198527

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FHIP (FHIP1B Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   silent mutations in codons 353 and 651 as well as…

   Promoter

   ADH1

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOBD2

   Plasmid

   #196826

   Purpose

   Yeast two-hybrid ; Gal4 DNA binding domain

   Depositor

   Stan Fields

   Article

   Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

   Type

   Empty backbone

   Use

   Tags

   Gal4 DNA binding domain

   Expression

   Yeast

   Mutation

   Promoter

   ADH

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LT2_15_UPRpro_X2site

   Plasmid

   #194427

   Purpose

   Plasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein Response

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   UPRpro
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LT2_32_PyruEth_X2site

   Plasmid

   #194428

   Purpose

   Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   PyruEth
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LT2_33_PyruPro_X2site

   Plasmid

   #194429

   Purpose

   Plasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   PyruPro
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LT2_34_EthPro_X2site

   Plasmid

   #194430

   Purpose

   Plasmid for genome integration in X2 site expressing ResPro (Ethanol consumption Probe)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   EthPro
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LT1_75_Donor_Tag_RPL13A-mTurq2

   Plasmid

   #194415

   Purpose

   Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   mTurquoise2 CDS
   

   Use

   Synthetic Biology; Used as donor dna in genome in…

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG306-pTet07-leuYFP[CTT]-nLuc

    Plasmid

    #199757

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[CTT]

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    leuYFP[CTT]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG306-pTet07-leuYFP[CTC]-nLuc

    Plasmid

    #199758

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[CTC]

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    leuYFP[CTC]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

    Plasmid

    #199760

    Purpose

    Elongation reporter construct to quantify elongation duration of 6_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-6xArg[CGA]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

    Plasmid

    #199761

    Purpose

    Elongation reporter construct to quantify elongation duration of 6_AGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-6xArg[AGA]-cLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C-SWAT 6FLAG

    Plasmid

    #182509

    Purpose

    C-SWAT compatible vector for C-terminal tagging with 6 FLAG

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    6 FLAG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C-SWAT IAA17-6FLAG (no NLS)

    Plasmid

    #193322

    Purpose

    C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    IAA17
    

    Use

    Tags

    6FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. M2C5

    Plasmid

    #188606

    Purpose

    Minimal Conjugative Plasmid 2

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. M1C1

    Plasmid

    #188609

    Purpose

    Minimal Conjugative Plasmid 1

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. M4C16

    Plasmid

    #188610

    Purpose

    Minimal Conjugative Plasmid 4

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. M4C19

    Plasmid

    #188611

    Purpose

    Minimal Conjugative Plasmid 4

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p426-Cup1p-FusionRed

    Plasmid

    #188392

    Purpose

    Cu2+-dependent expression of red fluorescent protein in yeast cells

    Depositor

    Michihiko Kataoka

    Article

    Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

    Insert

    FusionRed
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1 promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge