8,126 results

1. C-SWAT 3MYC SMASh

   Plasmid

   #182525

   Purpose

   C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL).

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   SMASh Tag
   

   Tags

   3MYC

   Expression

   Yeast

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. C-SWAT GFP

   Plasmid

   #182510

   Purpose

   C-SWAT compatible vector for C-terminal tagging with GFP

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   GFP
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. C-SWAT GFP DAmP

   Plasmid

   #182511

   Purpose

   C-SWAT compatible vector for C-terminal tagging with GFP DAmP. GFP is immediately followed by the Hygromycin B (Hph) resistance cassette.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   GFP
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. C-SWAT GFP CL1 DAmP

   Plasmid

   #182513

   Purpose

   C-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   CL1 and DAmP
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PKC1-2GFP-ter-TRP1

   Plasmid

   #184773

   Purpose

   Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   PKC1 (PKC1 Budding Yeast)
   

   Tags

   2EGFP

   Expression

   Yeast

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPG-GPD-TRPC

   Plasmid

   #190113

   Purpose

   Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2016-N

   Plasmid

   #190044

   Purpose

   Backbone vector for generation of BiFC plasmids

   Depositor

   Patrick Van Dijck

   Article

   Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   Met3

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2016-C

   Plasmid

   #190045

   Purpose

   Backbone vector for generation of BiFC plasmids

   Depositor

   Patrick Van Dijck

   Article

   Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   Met3

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGS_591

   Plasmid

   #160653

   Purpose

   Expresses OLE1 under the control of the yeast Sup35 promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   YGL055W (OLE1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   Sup35 promoter

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG25-1199 cDNA

    Plasmid

    #160379

    Purpose

    N1199 narnavirus cDNA

    Depositor

    John McCusker

    Insert

    N1199
    

    Expression

    Yeast

    Promoter

    NA

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PJD1223

    Plasmid

    #160390

    Purpose

    Encodes L-A cDNA for curing L-A virus and Parent for pJD1223-NAT

    Depositor

    John McCusker

    Insert

    L-A Gag-Pol
    

    Expression

    Yeast

    Promoter

    TRP1

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. N1199 launching

    Plasmid

    #160386

    Purpose

    Expression of N1199 narnavirus ORF under PGK1 promoter with a 3' HDV ribozyme sequence

    Depositor

    John McCusker

    Insert

    N1199
    

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGAL1-VP1 (LEU2)

    Plasmid

    #182480

    Purpose

    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. deltaVP1d63C + VP2C-GFPdeg

    Plasmid

    #182481

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-GFPdeg
    deltaVP1d63C
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. mRuby3 only VLP

    Plasmid

    #182429

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-mRuby3
    deltaVP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Linked mRuby3 VLP

    Plasmid

    #182430

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-GFP-mRuby3
    deltaVP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Coexpressed mRuby3 VLP

    Plasmid

    #182431

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-GFP
    deltaVP1
    VP2C-mRuby3
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1, GAL10, and GAL7

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Linked VLP NES

    Plasmid

    #182432

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Coexpressed VLP NES

    Plasmid

    #182433

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1, GAL10, and GAL7

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Linked VLP NES (M)

    Plasmid

    #182434

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(M)-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge