8,060 results

1. pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

   Plasmid

   #178999

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

   Plasmid

   #178998

   Purpose

   Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)

   Plasmid

   #178997

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUG36-NES-EGFP-cPHx3

   Plasmid

   #183668

   Purpose

   PtdIns(3,4)P2 biosensor for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Yeast

   Mutation

   Amino acids 169-329 (tandem trimer)

   Promoter

   MET25

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUG34-NES-EGFP-cPHx3

   Plasmid

   #183669

   Purpose

   PtdIns(3,4)P2 biosensor for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Yeast

   Mutation

   Amino acids 169-329 (tandem trimer)

   Promoter

   MET25

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pESC-LEU-IpgD

   Plasmid

   #183672

   Purpose

   Inducible Shigella flexneri IpgD for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pESC-LEU-IpgD(C439S)

   Plasmid

   #183673

   Purpose

   Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C439S

   Promoter

   GAL1

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

   Plasmid

   #178994

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR)

   Plasmid

   #178993

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

    Plasmid

    #178992

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

    Plasmid

    #178990

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEDJ506

    Plasmid

    #177274

    Purpose

    ADH1t:HIS3_23∆29-XII-5 :<-PGK1p

    Depositor

    Michael Jensen

    Article

    Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

    Insert

    ADH1t:HIS3_23∆29-XII-5 :<-PGK1p
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    PGK1

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pL-mWasabi

    Plasmid

    #176552

    Purpose

    Expression of mWasabi for auxotrophic selection in the absence of leucine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    mWasabi
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pM-eCFP

    Plasmid

    #176558

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of methionine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    eCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pH-TagRFP657

    Plasmid

    #176560

    Purpose

    Expression of TagRFP657 for auxotrophic selection in the absence of lysine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    TagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pM-TagRFP657

    Plasmid

    #176563

    Purpose

    Expression of TagRFP657 for auxotrophic selection in the absence of methionine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    TagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGB-Co-NF-kB

    Plasmid

    #180206

    Purpose

    Expression GAL4-fusion of Co-NF-kB in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kB
    

    Use

    Tags

    GAL4 (1-147) fusion

    Expression

    Yeast

    Mutation

    aa 2-1224

    Promoter

    AdhI

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGB-As-NF-kB2

    Plasmid

    #180210

    Purpose

    Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    As-NF-kB2
    

    Use

    Tags

    GAL4 (aa 1-147) fusion protein

    Expression

    Yeast

    Mutation

    aa 2-502

    Promoter

    AdhI

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJL32

    Plasmid

    #174066

    Purpose

    Encodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system.

    Depositor

    Albert Keung

    Article

    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

    Insert

    pGal1-LacO-3NLS-CIB1-VP16-tADH1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    pGal1

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GLuc VLP

    Plasmid

    #182213

    Purpose

    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).

    Depositor

    Claudia Vickers

    Inserts

    VP2C-GLuc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge