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8,240 results

Showing: 5941 - 5960 of 8240 results

  1. pMM4_14L

    Plasmid
    #183063
    Purpose
    Plasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.
    Inserts
    mCherry
    sfpHluorin
    BM3R1-HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Promoter
    RET2, Synthetic promoter, and TDH3
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183063-plasmid-map-sequence-id-363423

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  5. pIT6EG7mlh

    Plasmid
    #185887
    Purpose
    In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (23 copy).
    Depositor
    Claudia Vickers
    Insert
    see comments
    Expression
    Yeast
    Mutation
    ERG20 F96W N127W
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185887-plasmid-map-sequence-id-370841

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  6. pIPKAH

    Plasmid
    #185869
    Purpose
    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)
    Depositor
    Claudia Vickers
    Article
    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2
    Insert
    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185869-plasmid-map-sequence-id-370814

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  7. pIALD2HMGr

    Plasmid
    #185867
    Purpose
    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae
    Depositor
    Claudia Vickers
    Article
    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2
    Insert
    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185867-plasmid-map-sequence-id-370811

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  13. pIT7A6

    Plasmid
    #185896
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185896-plasmid-map-sequence-id-370384

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  14. pILAC2

    Plasmid
    #185889
    Purpose
    In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    see comments
    Expression
    Yeast
    Mutation
    ERG20 F96C; Xd.CRtYB E83K
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185889-plasmid-map-sequence-id-370860

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  15. pIT6EG7m

    Plasmid
    #185885
    Purpose
    A backbone plasmid for in vivo gene amplification (HamAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185885-plasmid-map-sequence-id-370833

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  16. pINER4R

    Plasmid
    #185884
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185884-plasmid-map-sequence-id-370832

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  17. pILGFP5EG3

    Plasmid
    #185878
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185878-plasmid-map-sequence-id-370821

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  18. pILGFP4BG6

    Plasmid
    #185877
    Purpose
    A parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locus
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185877-plasmid-map-sequence-id-370820

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  19. pILGFP3AE4

    Plasmid
    #185873
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185873-plasmid-map-sequence-id-370817

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  20. pILGFP3A5C

    Plasmid
    #185872
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185872-plasmid-map-sequence-id-370816

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Showing: 5941 - 5960 of 8240 results