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KBB11

Plasmid

#185422

Purpose

Full-length KAP60/SRP1

Depositor

Ken Belanger, Laura Davis

Publication

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

KAP60

Expression

Yeast

Promoter

endogenous

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Ken Belanger, Laura Davis

Publication

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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LDB59

Plasmid

#185436

Purpose

NUP1 CEN URA3 plasmid

Depositor

Ken Belanger, Laura Davis

Publication

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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KBB394

Plasmid

#185086

Purpose

NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

Depositor

Ken Belanger

Publication

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

Nma111 KKR 9-11 AAA; KRK 28-30 AAA

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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KBB349

Plasmid

#185080

Purpose

rfa2D248 truncation fused to GFP

Depositor

Ken Belanger

Publication

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA2

Tags

GFP

Expression

Yeast

Mutation

Rfa2 truncation aa 1-247

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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KBB353

Plasmid

#185081

Purpose

Swi6M-GFP expressing N-term 181 amino acids of Swi6

Depositor

Ken Belanger

Publication

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation aa 1-181

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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KBB484

Plasmid

#185132

Purpose

SWI6-GFP full length under control of GAL1 promotion

Depositor

Ken Belanger

Publication

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB486

Plasmid

#185133

Purpose

Full length CRM1 fused to binding domain of two-hybrid plasmid

Depositor

Ken Belanger

Publication

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

CRM1

Expression

Yeast

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB491

Plasmid

#185134

Purpose

Full length SWI6 fused to activation domain of two-hybrid plasmid

Depositor

Ken Belanger

Publication

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Expression

Yeast

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV492

Plasmid

#177700

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Publication

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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I282L

Plasmid

#184308

Purpose

To study dynamics of G-actin using NMR spectroscopy.

Depositor

Michael Rosen

Publication

Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

Insert

Act5C (Act5C Fly)

Tags

B-Thymosin followedby hexahistidine tag

Expression

Yeast

Mutation

I282L

Promoter

AOX1

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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I175L

Plasmid

#184322

Purpose

To study dynamics of G-actin using NMR spectroscopy.

Depositor

Michael Rosen

Publication

Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

Insert

Act5C (Act5C Fly)

Tags

Hexahistidine followed by B-thymosin

Expression

Yeast

Mutation

I175L

Promoter

AOX1

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-As-NF-kB1

Plasmid

#180209

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB1 protein in yeast

Depositor

Thomas Gilmore

Publication

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-409

Promoter

AdhI

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-Co-RHD

Plasmid

#180207

Purpose

Expresses GAL4 fusion of Capsaspora NF-kB Rel Homology Domain sequences in yeast

Depositor

Thomas Gilmore

Publication

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-RHD

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-581

Promoter

AdhI

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-DIPP3A-HA

Plasmid

#183948

Purpose

Expresses human HA-tagged DIPP3A from yeast GPD promoter

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

DIPP3A (NUDT10 Human)

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-DIPP2-HA

Plasmid

#183947

Purpose

Expresses human HA-tagged DIPP2 from yeast GPD promoter

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

DIPP2 (NUDT4 Human)

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-PPK(mutant)

Plasmid

#183942

Purpose

Expresses catalytic mutant of E. coli PPK from yeast GPD promoter

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

PPK(mutant) (ppk E. coli )

Expression

Yeast

Mutation

mutations in His435, His454, and His592 to Alanine

Promoter

GPD

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-DIPP3B-HA

Plasmid

#183949

Purpose

Expresses human HA-tagged DIPP3B from yeast GPD promoter

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

DIPP3B (NUDT11 Human)

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRS415-GPD-PPK

Plasmid

#183941

Purpose

Expresses E. coli PPK from yeast GPD promoter

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

PPK (ppk E. coli)

Expression

Yeast

Mutation

WT

Promoter

GPD

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-HA-PPK(mutant)

Plasmid

#183944

Purpose

Expresses HA-tagged catalytic mutant of E. coli PPK from yeast GPD promoter

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

PPK(mutant) (ppk E. coli)

Tags

HA

Expression

Yeast

Mutation

mutations in His435, His454, and His592 to Alanine

Promoter

GPD

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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