8,388 results
-
Plasmid#139421PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.DepositorInsertCitrine
ExpressionYeastPromoterTEF1Available SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-ProSgH
Plasmid#163974PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoterDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPAP003
Plasmid#153490PurposeLevel 2 integrative plasmid for stable expression in P. pastorisDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20F
Plasmid#145789PurposemPing Yeast Transposition AssayDepositorInsertmmPing20F
ExpressionYeastMutationInsertion of the FMV enhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20B
Plasmid#145790PurposemPing Yeast Transposition AssayDepositorInsertmmPing20B
ExpressionYeastMutationInsertion of the b-Conglycin EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A NL60
Plasmid#145791PurposemPing Yeast Transposition AssayDepositorInsertNL60
ExpressionYeastMutationInsertion of the OCS EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A 2xE
Plasmid#145792PurposemPing Yeast Transposition AssayDepositorInsert2xE
ExpressionYeastMutationInsertion of 2 copies of the CMV EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A 4xE
Plasmid#145793PurposemPing Yeast Transposition AssayDepositorInsert4XE
ExpressionYeastMutationInsertion of 4 copies of the CMV EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG-altTAG
Plasmid#158146PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG
Plasmid#158145PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BYG
Plasmid#158144PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertBFP-GFP fragment (wild-type)
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p-FAPB2.3.6
Plasmid#158142PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertFAPB2.3.6
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GXB
Plasmid#160926PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)DepositorInsertsBFP
sfGFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG
Plasmid#158132PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the first serine residue…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG2
Plasmid#158134PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the second serine residu…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG3
Plasmid#158136PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the third alanine residu…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG4
Plasmid#158137PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the third serine residue…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p-FAPB2.3.6L1TAG
Plasmid#158143PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertFAPB2.3.6L1TAG
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165V
Plasmid#158965PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165V
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165R
Plasmid#158966PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165R
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only