8,126 results

1. pDDGFP-Leu2d-GgKDELR2 R47A

   Plasmid

   #123620

   Purpose

   Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Arg47Ala

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDDGFP-Leu2d-GgKDELR2 R47K

   Plasmid

   #123621

   Purpose

   Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Arg47Lys

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDDGFP-Leu2d-GgKDELR2 E127A

   Plasmid

   #123622

   Purpose

   Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Glu127Ala

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDDGFP-Leu2d-GgKDELR2 E127Q

   Plasmid

   #123623

   Purpose

   Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Glu127Gln

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDDGFP-Leu2d-GgKDELR2 Y158F

   Plasmid

   #123624

   Purpose

   Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Tyr158Phe

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDDGFP-Leu2d-GgKDELR2 R159A

   Plasmid

   #123625

   Purpose

   Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Arg159Ala

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICE LYS2-URA3

   Plasmid

   #122890

   Purpose

   LYS2-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCuHA-ATG13[3SD](426)

   Plasmid

   #122077

   Purpose

   Expressing HA-ATG13[3SD]

   Depositor

   Daniel Klionsky

   Article

   Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

   Insert

   ATG13
   

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Serine 646 to aspartic acid; Serine 649 to aspart…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSM002

   Plasmid

   #122936

   Purpose

   Expression of the marked mRNA under the control of substitutional promoters.

   Depositor

   Attila Becskei

   Article

   Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

   Insert

   YML043C (Marked)
   

   Expression

   Yeast

   Mutation

   YML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATAC

   Promoter

   [tetO2]4inPgal1

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSM001

    Plasmid

    #122935

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YML029W (Marked)
    

    Expression

    Yeast

    Mutation

    YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEC005

    Plasmid

    #122939

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YML071C (Marked)
    

    Expression

    Yeast

    Mutation

    YML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGC

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSV_238

    Plasmid

    #122941

    Purpose

    Expression of the repressor to control the insertional promoter.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    rTetR.SUM1
    

    Expression

    Yeast

    Promoter

    Pret2

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSV_207

    Plasmid

    #122938

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YML070W (Marked)
    

    Expression

    Yeast

    Mutation

    YML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTA

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSV_243

    Plasmid

    #122940

    Purpose

    Expression of the marked mRNA under the control of insertional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YML029W (Marked)
    

    Expression

    Yeast

    Mutation

    YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

    Promoter

    Pyml029w-tetO(2)

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSM004

    Plasmid

    #122937

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YML065W (Marked)
    

    Expression

    Yeast

    Mutation

    YML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAAC

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSV_228

    Plasmid

    #122933

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YBR025C (Marked)
    

    Expression

    Yeast

    Mutation

    YBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTA

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSV_229

    Plasmid

    #122934

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YBR026C (Marked)
    

    Expression

    Yeast

    Mutation

    YBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAG

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS-ATG1(mut)(406)

    Plasmid

    #122069

    Purpose

    Expressing a mutant ATG1

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Codon changes without changing the amino acid seq…

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYZ292

    Plasmid

    #102686

    Purpose

    S. pombe gRNA entry vector with Cas9 - ScLEU2 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWS1859

    Plasmid

    #123065

    Purpose

    Positive regulator of Ste20

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Ste50 [3]
    

    Expression

    Yeast

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge