8,060 results

1. pRS424-TUB2 - human TUBB1 Cterminal tail

   Plasmid

   #60400

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRS424-TUB2 - human TUBB3 Cterminal tail

   Plasmid

   #60397

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C127S; TUB2 amino acids 429 - end, replaced with …

   Promoter

   GAL

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pRPR1_c2gRNA_RPR1t

   Plasmid

   #64380

   Purpose

   encodes c2 gRNA

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   c2 gRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pRPR1_g1gRNA_RPR1t

   Plasmid

   #64387

   Purpose

   encodes g1 gRNA

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   g1 gRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pRPR1_c7gRNA_RPR1t

   Plasmid

   #64385

   Purpose

   encodes c7 gRNA

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   c7 gRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pRPR1_c1gRNA_RPR1t

   Plasmid

   #64379

   Purpose

   encodes c1 gRNA

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   c1 gRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pRPR1_c5gRNA_RPR1t

   Plasmid

   #64383

   Purpose

   encodes c5 gRNA

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   c5 gRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Gal4pBS13-Pleum

   Plasmid

   #60332

   Purpose

   Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pJW1509

   Plasmid

   #62363

   Purpose

   pFA6a BirA-heh2 linker-SSH1::HIS5

   Depositor

   Jonathan Weissman

   Article

   Jan et al Science. 2014 Nov 7;346(6210):1257521. d

   Insert

   BirA-heh2-Ssh1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGAD-hPLEKHM1

    Plasmid

    #64145

    Purpose

    For yeast two hybrid

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Use

    Tags

    GAL4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pGAD-hRubicon

    Plasmid

    #64144

    Purpose

    For yeast two hybrid

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    GAL4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pNB786

    Plasmid

    #60151

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNB762

    Plasmid

    #60150

    Purpose

    Firefly luciferase (Promega) driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNB767

    Plasmid

    #60159

    Purpose

    Firefly luciferase (Promega) driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNB682

    Plasmid

    #60143

    Purpose

    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJW3.31

    Plasmid

    #62865

    Purpose

    Yeast two-hybrid bridge vector expressing PAA-1

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    PAA-1/Y3H
    

    Use

    Two-hybrid bridge vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRevLCav2deltaC

    Plasmid

    #60809

    Purpose

    to make truncation of yeast Rev3

    Depositor

    Youri Pavlov, Tahir Tahirov

    Article

    Siebler et al DNA Repair (Amst). 2014 May 9. pii: S156

    Insert

    REV3 (REV3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    rev3ΔC encodes for Rev3 lacking the C-terminus (a…

    Promoter

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRevLCav2FeS

    Plasmid

    #60808

    Purpose

    to disrupt iron sulfur cluster in yeast Rev3

    Depositor

    Youri Pavlov, Tahir Tahirov

    Article

    Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

    Insert

    REV3 (REV3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    contains 647 C-terminal amino acids and 335 bp of…

    Promoter

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Gal4pBS134-Pleum

    Plasmid

    #60339

    Purpose

    Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Gal4pBS3-Pleum

    Plasmid

    #60330

    Purpose

    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge