8,060 results

1. L284F Cus2p

   Plasmid

   #73059

   Purpose

   S. cerevisiae Cus2p gene with L284F Mutation

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Use

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Mutation

   Leucine 284 to Phenylalanine

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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2. D282N Cus2p

   Plasmid

   #73060

   Purpose

   S. cerevisiae Cus2p gene with D282N Mutation

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Use

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Mutation

   Asp 282 to Asn

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS313-prp8-S2197F

   Plasmid

   #89967

   Purpose

   S2197F point mutant

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   S2197F

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS313-prp8-H2387P

   Plasmid

   #89968

   Purpose

   H2387P point mutant

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H2387P

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS313-prp8-R2388G

   Plasmid

   #89969

   Purpose

   R2388G point mutant

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R2388G

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS314-prp16-1

   Plasmid

   #89972

   Purpose

   prp16-1 allele in TRP plasmid

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp16-1 allele (Y386D)

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS314-prp16-101

   Plasmid

   #89973

   Purpose

   prp16-101 allele in TRP plasmid

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp16-101 allele (G373S)

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHES936

   Plasmid

   #89199

   Purpose

   Expresses DIG1 under control of GAL1 promoter

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   RST1 (DIG1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSL3 - Brr2 del113 N1104L

   Plasmid

   #90253

   Purpose

   protein expression

   Depositor

   Christine Guthrie

   Article

   Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

   Insert

   BRR2
   

   Use

   Tags

   TAP

   Expression

   Yeast

   Mutation

   deleted first 113 amino acids and amino acid N110…

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSL7 - Brr2 del247 N1104L

    Plasmid

    #90257

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Use

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 247 amino acids and N1104L

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

11. pSL11 - Brr2 del422 N1104L

    Plasmid

    #90261

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Use

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 422 amino acids and amino acid N110…

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSL12 - Brr2 del422 Q904E

    Plasmid

    #90262

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Use

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 422 amino acids and amino acid Q904E

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSL13 - Brr2 del422 R1107L

    Plasmid

    #90263

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Use

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 422 amino acids and amino acid R110…

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHES942

    Plasmid

    #90408

    Purpose

    Expresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-SIC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Sub2-3 in pRS314

    Plasmid

    #90454

    Purpose

    yeast

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-3 mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Sub2-4 in pRS314

    Plasmid

    #90455

    Purpose

    yeast

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-4 mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Sub2-6 in pRS314

    Plasmid

    #90456

    Purpose

    yeast

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-6 mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV10.F3.loxP

    Plasmid

    #63200

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV10.K3.loxP

    Plasmid

    #63202

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV10.K6.loxP

    Plasmid

    #63203

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge