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pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)

Plasmid

#179005

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

Plasmid

#179004

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-pro-alphaf-Btl2

Plasmid

#117664

Purpose

Study secretion efficiency of Btl2

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf-Btl2

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSP-GM1

Plasmid

#64739

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al FEMS Yeast Res. 2012 Aug;12(5):598-607.

Type

Empty backbone

Expression

Yeast

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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BS-Ura3-GFP-Atg8

Plasmid

#69194

Purpose

Single copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

GFP-Atg8

Tags

EGFP

Expression

Yeast

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-mYFP-hphMX6

Plasmid

#105155

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-hphMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p404-BrdU-Inc

Plasmid

#71790

Purpose

BrdU-incorporating plasmid with selectable marker TRP1

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BYG

Plasmid

#158126

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB2904(XI-3 MarkerFree)

Plasmid

#73276

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pRS424GFP-2xPH(Osh2)

Plasmid

#36095

Depositor

Scott Emr

Article

Stefan et al Cell. 2011 Feb 4;144(3):389-401.

Insert

2xPH(Osh2) fusion

Tags

GFP

Expression

Yeast

Promoter

PRC1

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
p416-GPD-DIPP1-HA

Plasmid

#183946

Purpose

Expresses human HA-tagged DIPP1 from yeast GPD promoter

Depositor

Michael Downey

Insert

DIPP1 (NUDT3 Human)

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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416Gal-FUS-P525L-YFP

Plasmid

#29628

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

P525L

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pTH847-gst-optsc

Plasmid

#96908

Purpose

Plasmid for yeast expression of GST encoded by preferred codons

Depositor

Tobias von der Haar

Article

Jossé et al bioRxiv 200006

Insert

yeast codon optimised glutathione-S transferase

Tags

8xhistidine

Expression

Yeast

Promoter

TDH3

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pXP422

Plasmid

#26845

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pWS3865

Plasmid

#185742

Purpose

Sub-array backbone 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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ZJOM153

Plasmid

#133642

Purpose

Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

Vph1-2GFP (VPH1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-5FLAG-bleMX6

Plasmid

#19344

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

bleMX6

Expression

Yeast

Available Since

Sept. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pXP418

Plasmid

#26843

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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UASGAL-Pgal

Plasmid

#60338

Purpose

Contains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

Plasmid

#58438

Purpose

Expresses LexA-ER-Gal4AD from yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-Gal4AD

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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