8,086 results

1. p42Hyg 1-F GW

   Plasmid

   #58551

   Purpose

   Multicopy vector with Hygromycin resistance marker for single Gateway insertion

   Depositor

   Leonid Kruglyak

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWKS5

   Plasmid

   #139412

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   Citrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY2

   Plasmid

   #218115

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY3

   Plasmid

   #218116

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY3-LYS-IV

   Plasmid

   #218119

   Purpose

   LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pM

   Plasmid

   #64179

   Purpose

   harbours marker gene MET17

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Aug. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. ZJOM103

   Plasmid

   #133664

   Purpose

   Integration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYX223-mtGFP

   Plasmid

   #45051

   Depositor

   Benedikt Westermann

   Article

   Westermann et al Yeast. 2000 Nov;16(15):1421-7.

   Insert

   mtGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

9. YIp128-CUP1-NUbo-GFP-His6

   Plasmid

   #212790

   Purpose

   Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-GFP-His6
   

   Use

   Integrative vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LHP1463 - INP52

    Plasmid

    #32432

    Depositor

    Linda Hicke

    Article

    Stamenova et al J Biol Chem. 2004 Apr 16;279(16):16017-2

    Insert

    INP52 (INP52 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Lac

    Available Since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pRS426-GFP-Osh1-PH

    Plasmid

    #71372

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh1-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa267-388

    Promoter

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pPTK002-2-pGAP

    Plasmid

    #84961

    Purpose

    Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Glyceraldehyde-3-phosphate dehydrogenase promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    BsaI site removed (1805 c to g)

    Promoter

    Non

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGALSc104(WT)

    Plasmid

    #1146

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    HSP104 (HSP104 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTCW007

    Plasmid

    #83525

    Purpose

    pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

    Depositor

    Claudia Vickers

    Article

    Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

    Insert

    pFUS1J2-mfalpha1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pgRNA-backbone

    Plasmid

    #172517

    Purpose

    Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

    Depositor

    Leonid Kruglyak

    Article

    Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

    Insert

    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHES822

    Plasmid

    #89193

    Purpose

    Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-YFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179051

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-RPC19-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p414GPD-3xFLAG-GW

    Plasmid

    #63780

    Purpose

    yeast expression vector p414GPD modified such that it is Gateway converted and introduces a 3xFLAG tag at the N-terminus of the insert

    Depositor

    Susan Baserga

    Article

    McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. p426 25Q GAL

    Plasmid

    #1187

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pILGFPH7

    Plasmid

    #83554

    Purpose

    Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    ADH2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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