8,436 results
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Plasmid#133672PurposeIntegration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.DepositorAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only
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ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZac-STE2-1D4
Plasmid#133437PurposeMutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAPZ-STE2-1D4
Plasmid#133423PurposeYeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
ExpressionYeastMutationC59S, C252A. No alpha factor secretion signal in …Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0327
Plasmid#133488PurposeExpression of nucleo-cytoplasmic sfGFPDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)
ExpressionYeastAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAV0413
Plasmid#133470PurposeIntegration into fission yeast genome at his5 locusDepositorTypeEmpty backboneExpressionYeastAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0357
Plasmid#133469PurposeIntegration into fission yeast genome at lys3 locusDepositorTypeEmpty backboneExpressionYeastAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0612
Plasmid#133505PurposeExpression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitCDepositorInsertpAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
ExpressionYeastAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0526
Plasmid#133500PurposeExpression of sfGFP targeted to the nucleus with one NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX
ExpressionYeastAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0785
Plasmid#133507PurposeExpression of mCherry tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX
ExpressionYeastAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0620
Plasmid#133506PurposeExpression of eGFP tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
ExpressionYeastAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0478
Plasmid#133498PurposeExpression of sfGFP targeted to the nucleus with two NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX
ExpressionYeastAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0782
Plasmid#133514PurposeExpression of sfGFP tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
ExpressionYeastAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK221
Plasmid#129249Purposereverse yTRAP, tetR-responsive Neon reporterDepositorInsertpADH1(tetO2)-mNeonGreen
ExpressionYeastPromoterADH1Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesExpressionYeastPromoterPADH1Available sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
prs316 CUP-GFP
Plasmid#128052PurposeYeast expression vector for N terminal GFP fusionsDepositorTypeEmpty backboneTagsGFPExpressionYeastAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-LEU2
Plasmid#122909PurposeLYS2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA149
Plasmid#128524PurposeExpresses mutant Ber e 1 in PichiaDepositorInsertmutant Ber e 1
ExpressionYeastPromoterAOX1Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-NpgA
Plasmid#126731Purposeyeast plasmid overexpressing NpgADepositorInsertnpgA
Tags6xHisExpressionYeastMutationG951A,C957AAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only