8,432 results

Showing: 1901 - 1920 of 8432 results

Results per page:

Sort results by:

pRS267

Plasmid

#122380

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Publication

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Expression

Yeast

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ293

Plasmid

#102687

Purpose

S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ294

Plasmid

#102688

Purpose

S. pombe gRNA entry vector with Cas9 - KanMX marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ300

Plasmid

#102689

Purpose

S. pombe gRNA entry vector with Cas9 - Sp his3 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ301

Plasmid

#102690

Purpose

S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMG13

Plasmid

#122932

Purpose

Expression of the regulator tTA.

Depositor

Attila Becskei

Publication

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

tTA

Expression

Yeast

Promoter

Pcln3

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHES609 [prNOp7-PBAC]

Plasmid

#103017

Purpose

A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

Depositor

Hana El-Samad

Publication

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

bPAC

Expression

Yeast

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW mScarletI-T2A-mTurquoise2

Plasmid

#118430

Purpose

Produces equimolar expression of mScarletI and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mScarletI-T2A-mTurquoise2

Expression

Yeast

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW ymVenus

Plasmid

#118454

Purpose

Overexpression of yeast codon-optimized mVenus

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUC2::GNSI (NrsR)

Plasmid

#121537

Purpose

This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.

Depositor

Alexey Merz

Publication

Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

Inserts

CYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR

Tags

mGFP5

Expression

Yeast

Mutation

M109I (Naturally occurring SNP) and The first 21 …

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHES822

Plasmid

#89193

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Expression

Yeast

Promoter

pZ

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

Plasmid

#116884

Purpose

FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

Depositor

Brian Kuhlman

Publication

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RLR)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pPICZ-pre-pro-alphaf-E2-Crimson

Plasmid

#117658

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Publication

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJY4092

Plasmid

#113334

Purpose

Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Insert

codon-optimized Hermes transposase

Expression

Yeast

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Gma-pCAMBIA-1301-STTM171

Plasmid

#84080

Purpose

Disturb the expression of miR171 in soybean

Depositor

Guiliang Tang

Publication

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR171

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCdeltaSS-26X

Plasmid

#108218

Purpose

Yeast reporter gene with glucocorticoid response element (GRE) upstream of CYC1 promoter driving lacZ expression

Depositor

Didier Picard, Keith Yamamoto

Publication

Schena et al Science. 1988 Aug 19. 241(4868):965-7.

Insert

GRE-CYC1-lacZ

Expression

Yeast

Promoter

CYC1

Available since

Sept. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB2176

Plasmid

#113395

Purpose

Ty3-IN-GFP; alpha factor inducible

Depositor

Suzanne Sandmeyer

Publication

Beliakova-Bethell et al RNA. 2006 Jan;12(1):94-101. doi: 10.1261

Insert

Ty3 with integrase-green fluorescent protein

Tags

IN-GFP

Expression

Yeast

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMF2

Plasmid

#112819

Purpose

Expresses wild-type Rpb1 in yeast cells

Depositor

Stephen Fuchs

Publication

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge