9,746 results

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pLVX-hSyn-Galpha-i1 ONE-GO

Plasmid

#204333

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i1 ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-q ONE-GO

Plasmid

#204336

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-q ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-13 ONE-GO

Plasmid

#204337

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-13 ONE-GO (GNA13 Human)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-i3 G183S ONE-GO

Plasmid

#204338

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3 G183S ONE-GO

Use

Lentiviral

Mutation

G183S mutation in Galpha-i3 (renders it RGS insen…

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204816

Purpose

Bacterial expresison of Enterovirus A71 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

EV A71 3C protease

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGL4.84(hRlucCP/Puro)

Plasmid

#212935

Purpose

RapidResponse(TM) Renilla luciferase vector for investigating regions controlling transcription

Depositor

Pete Stecha

Type

Empty backbone

Use

Luciferase

Mutation

WT

Available Since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-miRFP670nano3-Blast-H3C2

Plasmid

#207784

Purpose

Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1α-Cre-2A-miRFP670nano3

Plasmid

#207747

Purpose

Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-miRFP670nano3

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-moxGFP-Zeo

Plasmid

#207732

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-V5-Blast

Plasmid

#207719

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-sTagRFP-Puro

Plasmid

#207723

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-mScarlet

Plasmid

#207700

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-moxGFP-Blast

Plasmid

#207710

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Blast-V5

Plasmid

#207686

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Blast-miniTurbo

Plasmid

#207681

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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p3xFLAG-CMV14-GINIP

Plasmid

#204845

Purpose

Express 3x FLAG tagged-GINIP in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

PHF24 (KIAA1045 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Sept. 14, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCS-lpp0.2-H38A

Plasmid

#201788

Purpose

mutant of PadR; transcriptional regulator can respond to p-coumaric acid

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PadR (padR Bacillus subtilis)

Expression

Bacterial

Mutation

H38A

Available Since

July 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pZE-PpadC-yveF-yveG-egfp

Plasmid

#201791

Purpose

the promoter can be recognized by PadR

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PpadC-yveF-yveG

Expression

Bacterial

Mutation

WT

Available Since

July 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDSP32

Plasmid

#199374

Purpose

Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression

Depositor

Hang Lu

Insert

NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS

Expression

Bacterial

Mutation

no mutations, except added subcellular localizati…

Available Since

April 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDSP1

Plasmid

#199384

Purpose

Kanamycin resistant cloning vector backbone for pDSP23, pDSP31-33, pHJL1-4, pSiM1-4

Depositor

Hang Lu

Type

Empty backbone

Expression

Bacterial

Mutation

Cloning vector backbone

Available Since

April 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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