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GAG-PKB-pcDNA3
(Plasmid #24651)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24651 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKB
  • Species
    H. sapiens (human)
  • Entrez Gene
    AKT1 (a.k.a. AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Tag / Fusion Protein
    • GAG (N terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

P106S mutation in GAG and S2D, V45L, D46E, A50S, K112R, E117T, S126G, D127E mutations detected in PKB Addgene quality control sequence. Author reports that these mutations do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GAG-PKB-pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 24651 ; http://n2t.net/addgene:24651 ; RRID:Addgene_24651)
  • For your References section:

    Protein kinase B regulates T lymphocyte survival, nuclear factor kappaB activation, and Bcl-X(L) levels in vivo. Jones RG, Parsons M, Bonnard M, Chan VS, Yeh WC, Woodgett JR, Ohashi PS. J Exp Med. 2000 May 15. 191(10):1721-34. 10.1084/jem.191.10.1721 PubMed 10811865