|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24651||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
/ Fusion Protein
- GAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
P106S mutation in GAG and S2D, V45L, D46E, A50S, K112R, E117T, S126G, D127E mutations detected in PKB Addgene quality control sequence. Author reports that these mutations do not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GAG-PKB-pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 24651 ; http://n2t.net/addgene:24651 ; RRID:Addgene_24651)
For your References section:Protein kinase B regulates T lymphocyte survival, nuclear factor kappaB activation, and Bcl-X(L) levels in vivo. Jones RG, Parsons M, Bonnard M, Chan VS, Yeh WC, Woodgett JR, Ohashi PS. J Exp Med. 2000 May 15. 191(10):1721-34. 10.1084/jem.191.10.1721 PubMed 10811865