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pShuttle

Plasmid

#192871

Purpose

Gateway compatible vector with BsaI cloning site

Depositor

Thorsten Stiewe

Publication

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Type

Empty backbone

Use

Gateway-compatible cloning vector

Available since

Dec. 12, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJBJZ_001

Plasmid

#155357

Purpose

Gentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Publication

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJBJZ_003

Plasmid

#155358

Purpose

Rifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Publication

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJBJZ_006

Plasmid

#155359

Purpose

Zeomycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Publication

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pIT6EG7ml

Plasmid

#185886

Purpose

In vivo gene amplification (HapAmp) of limonene synthetic module at RPL25 locus (14 copy).

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

see comments

Expression

Yeast

Mutation

ERG20 F96W N127W

Available since

Sept. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pINER5

Plasmid

#185895

Purpose

In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2

Expression

Yeast

Mutation

None

Available since

Aug. 25, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CD Ugi1

Plasmid

#177194

Purpose

Greengate entry vector pGGC000 modified to contain bladderwort insulator 1

Depositor

Wayne Parrott

Publication

Park et al bioRxiv 2021.11.04.467280

Insert

Utricularia gibba insulator 1

Expression

Bacterial

Available since

June 28, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CD Ugi3

Plasmid

#177195

Purpose

Greengate entry vector pGGC000 modified to contain bladderwort insulator 3

Depositor

Wayne Parrott

Publication

Park et al bioRxiv 2021.11.04.467280

Insert

Utricularia gibba insulator 3

Expression

Bacterial

Available since

June 28, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-mScarlet-MAVS-5'UTR

Plasmid

#184639

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTR

Depositor

Hao Wu

Publication

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Expression

Mammalian

Available since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1023 (pDS-InR1)

Plasmid

#183130

Purpose

Expresses dsRNA targeting the InR1 gene from Apis mellifera

Depositor

Jeffrey Barrick

Publication

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Fragment of InR1 gene

Use

Synthetic Biology

Available since

May 19, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK150

Plasmid

#183126

Purpose

BTK Type 2 promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression

Use

Synthetic Biology

Available since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5_Ptet

Plasmid

#179336

Purpose

gRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos4_Ptet

Plasmid

#179335

Purpose

gRNA Position Vector 4, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5b2_Ptet

Plasmid

#179342

Purpose

gRNA Position Vector 5b2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Publication

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDRf1-SweetTrac1

Plasmid

#169083

Purpose

Expression of SweetTrac1 biosensor in yeast

Depositor

Lily S Cheung

Publication

Park et al Proc Natl Acad Sci U S A. 2022 Jan 25;11

Insert

SweetTrac1

Expression

Yeast

Promoter

PMA1

Available since

Feb. 4, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MS2_SC2_S_Omicron

Plasmid

#180229

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)

Depositor

Paul Freemont

Inserts

Maturation Protein
Coat Protein Dimer
S gene (S SARS-CoV-2)

Expression

Bacterial

Mutation

Omicron (Amino Acid 319-869)

Promoter

T7

Available since

Jan. 28, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL1:GUS

Plasmid

#175557

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Publication

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL1

Tags

GUS

Expression

Plant

Promoter

GOXL1

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2124

Plasmid

#178791

Purpose

Prgef-1 EGL-3 unc-54 3' UTR C.elegans pan-neural expression of EGL-3

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

EGL-3 (egl-3 Nematode)

Expression

Worm

Promoter

Prgef-1

Available since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pJH2930

Plasmid

#175283

Purpose

Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Expression

Worm

Promoter

Pges-1

Available since

Dec. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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