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pGBW-m4046521

Plasmid

#145473

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp11

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Citrine-CathespinB-6

Plasmid

#56554

Purpose

Localization: Lysosomes, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Insert

CathespinB

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
UBH12 (3CLZ)

Plasmid

#25220

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ubh12.414.617:B9 (UHRF1 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 12, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4046696

Plasmid

#153882

Purpose

Yeast expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mTurquoise-Fibrillarin-7

Plasmid

#55552

Purpose

Localization: Nucleoli, Excitation: 434, Emission: 434

Depositor

Michael Davidson

Insert

Fibrillarin (FBL Human)

Tags

mTurquoise

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133373

Plasmid

#152125

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp10

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mCitrine-Rab4a-7

Plasmid

#56565

Purpose

Localization: Ras GTPase, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Insert

Rab4a (RAB4A Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEos3.2-Telethonin-C-18

Plasmid

#62770

Purpose

Localization: Scaffold, C1 Cloning Vector, Excitation:507 / 572, Emission:516 / 580

Depositor

Michael Davidson

Insert

Telethonin

Tags

mEos3.2

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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miniSOG-Keratin-17

Plasmid

#57770

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Keratin (KRT18 Human)

Tags

miniSOG

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC4A9

Plasmid

#132216

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A9 (SLC4A9 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-A1:hphMX4

Plasmid

#134425

Purpose

PCR-based C-terminal A1 tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

A1

Use

Pcr-based tagging in yeast

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mVenus-VASP-5

Plasmid

#56624

Purpose

Localization: Actin/Focal Adhesions, Excitation: 515, Emission: 528

Depositor

Michael Davidson

Insert

VASP

Tags

mVenus

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mGeos-M-Actin-C-18

Plasmid

#57523

Purpose

Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Tags

mGeos-M

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mAmetrine-Profilin-10

Plasmid

#56549

Purpose

Localization: Cytoskeleton, Excitation: 406, Emission: 526

Depositor

Michael Davidson

Insert

Profilin

Tags

mAmetrine

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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KIFC1

Plasmid

#25253

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KIFC1:G361-C715 (KIFC1 Human)

Use

Baculovirus expression

Tags

His

Mutation

Contains amino acids G361-C715

Promoter

polyhedrin

Available Since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
UBE2G1

Plasmid

#25614

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

UBE2G1 (UBE2G1 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 31, 2010

Availability

Industry, Academic Institutions, and Nonprofits
mEos4a-LaminA-C-18

Plasmid

#57497

Purpose

Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

LaminA (LMNA Human)

Tags

mEos4a

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4133409

Plasmid

#152854

Purpose

Mammalian expression plasmid for SARS-CoV-2 endoRNAse

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1665

Plasmid

#29289

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple193 (SLC6A3 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Sept. 1, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4252867

Plasmid

#153626

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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