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9,745 results

Showing: 6401 - 6420 of 9745 results

  2. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224697-plasmid-map-sequence-id-444276

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  3. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224698-plasmid-map-sequence-id-444264

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  9. pcDNA3.1-GSDMD_1-270

    Plasmid
    #218229
    Purpose
    Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-270
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218229-plasmid-map-sequence-id-430564

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  10. pcDNA3.1-GSDMD_29-484

    Plasmid
    #218231
    Purpose
    Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 29-484
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218231-plasmid-map-sequence-id-436177

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  11. pcDNA3.1-GSDMD_29-270

    Plasmid
    #218232
    Purpose
    Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 29-270
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218232-plasmid-map-sequence-id-430566

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  12. pAG CMV HA-EGFP Puro

    Plasmid
    #237882
    Purpose
    Mammalian HA-EGFP expression plasmid, control plasmid for Addgene #237879
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    EGFP
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237882-plasmid-map-sequence-id-471570

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  13. pAG CMV EGFP-HA Puro

    Plasmid
    #237883
    Purpose
    Mammalian EGFP-HA expression plasmid, control plasmid for Addgene #237880
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    EGFP
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237883-plasmid-map-sequence-id-469908

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  14. pAG CMV C-HA Puro

    Plasmid
    #237880
    Purpose
    Empty mammalian expression plasmid for cloning C-terminally HA-tagged proteins
    Depositor
    Meghan Rego, Addgene Research Program
    Type
    Empty backbone
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237880-plasmid-map-sequence-id-469913

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  15. pAG CMV EGFP Puro

    Plasmid
    #237884
    Purpose
    Mammalian EGFP expression plasmid, control plasmid for Addgene #237881
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237884-plasmid-map-sequence-id-469914

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  18. pPB.DEST-Illusia(YYEE)

    Plasmid
    #222594
    Purpose
    Piggybac expression vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation
    Depositor
    Johanna Ivaska
    Article
    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038
    Insert
    Illusia(YYEE)
    Use
    Transposon-based stable expression
    Expression
    Mammalian
    Mutation
    Mutations in the NPxY sites equivolent to Y783E, …
    Promoter
    CAG
    Available Since
    May 29, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222594-plasmid-map-sequence-id-450070

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  20. pPB.DEST-Illusia(Y783F)

    Plasmid
    #222596
    Purpose
    Piggybac expression vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylation
    Depositor
    Johanna Ivaska
    Article
    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038
    Insert
    Illusia(Y783F)
    Use
    Transposon-based stable expression
    Expression
    Mammalian
    Mutation
    Mutation in the NPxY(783) site equivolent to Y783…
    Promoter
    CAG
    Available Since
    May 29, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222596-plasmid-map-sequence-id-450084

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Showing: 6401 - 6420 of 9745 results