Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

9,627 results

Showing: 6801 - 6820 of 9627 results

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pHTC-HaloTag_CDC73:1-531

Plasmid

#210983

Purpose

Mammalian expression of full-length human CDC73. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CDC73:M1-F531 (CDC73 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_SMCR8:1-937

Plasmid

#210984

Purpose

Mammalian expression of full-length human SMCR8. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SMCR8:M1-I937 (SMCR8 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_SMCR8:1-937

Plasmid

#210985

Purpose

Mammalian expression of full-length human SMCR8. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SMCR8:M1-I937 (SMCR8 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_FBXW5:1-566

Plasmid

#210981

Purpose

Mammalian expression of full-length human FBXW5. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW5:M1-R566 (FBXW5 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_ACTB:1-375

Plasmid

#210971

Purpose

Mammalian expression of full-length human ACTB. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ACTB:M1-F375 (ACTB Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_DCAF12:1-453

Plasmid

#210967

Purpose

Mammalian expression of full-length human DCAF12. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_ACTB:1-375

Plasmid

#210970

Purpose

Mammalian expression of full-length human ACTB. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ACTB:M1-F375 (ACTB Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MERS Mpro - His6-SUMO with autoprocessing - MSAVLQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204814

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro (orf1ab MERS)

Expression

Bacterial

Mutation

MSAVLQ/SGLVKM

Available Since

Feb. 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Enterovirus A71 2A protease

Plasmid

#204812

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available Since

Feb. 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Blast-moxGFP-LMNB1

Plasmid

#207772

Purpose

Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

LMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Feb. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-Fld

Plasmid

#202181

Purpose

Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Fld terminator

Use

Synthetic Biology

Mutation

None

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1059

Plasmid

#209549

Purpose

BTK Type 5 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv 2023.09.19.558440

Insert

Downstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)

Use

Synthetic Biology

Available Since

Feb. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (T190I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204786

Purpose

Bacterial expression of Sars Cov 2 MPRO T190I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

T190I

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Sars Cov 2 MPRO R188S mutantSARS-CoV-2 Mpro protease (R188S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204785

Purpose

Bacterial expression of Sars Cov 2 MPRO R188S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

R188S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (Q189K mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204784

Purpose

Bacterial expression of Sars Cov 2 MPRO Q189K mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

Q189K

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (P132H mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204783

Purpose

Bacterial expression of Sars Cov 2 MPRO P132H mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

P132H

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (N142S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204782

Purpose

Bacterial expression of Sars Cov 2 MPRO N142S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

N142S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (M49T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204779

Purpose

Bacterial expression of Sars Cov 2 MPRO M49T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49T

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204777

Purpose

Bacterial expression of Sars Cov 2 MPRO M49I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M49I

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (G143S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204776

Purpose

Bacterial expression of Sars Cov 2 MPRO G143S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

G143S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

Showing: 6801 - 6820 of 9627 results

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