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MLKS2delKASHec

Plasmid

#130858

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Gateway Cloning

Tags

eGFP-FLAG-HA

Mutation

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHWBF07EC

Plasmid

#130828

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Use

Gateway Cloning

Tags

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHWBF09EC

Plasmid

#130829

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Use

Gateway Cloning

Tags

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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ECPK1D

Plasmid

#130830

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Use

Gateway Cloning

Tags

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHWBF07exp

Plasmid

#130832

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-N SARS-CoV-2 Omicron spike protein

Plasmid

#180779

Purpose

Sars-CoV-2 variant protein

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

MAC-N SARS-CoV-2 Omicron spike protein

Tags

MAC-tag

Expression

Mammalian

Mutation

S371L, H69-V70-, S373P, S375F, G339D, N211-, N440…

Available Since

Sept. 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-act1_full

Plasmid

#202172

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 full terminator

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC-GST-GINIP

Plasmid

#204844

Purpose

Express GST tagged-GINIP in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

PHF24 (KIAA1045 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-pyr_comp_sig_pep

Plasmid

#202132

Purpose

Level 0 plasmid containing a kozak consensus sequence and a pyrenoid compartment signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Pyrenoid compartment signal peptide

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 6, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0-EF-54730

Plasmid

#202163

Purpose

Level 0 plasmid containing a terminator sequence from gene 54730 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

54730 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 6, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-glna

Plasmid

#202183

Purpose

Level 0 plasmid containing a terminator sequence from gene 22357 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

glna terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-act1_tr

Plasmid

#202171

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 truncated terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-GPI1

Plasmid

#202124

Purpose

Level 0 plasmid containing the promoter from gene 23924 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

GPI1 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-fcpF

Plasmid

#202119

Purpose

Level 0 plasmid containing the promoter from gene 51230 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

fcpF promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-27877

Plasmid

#202110

Purpose

Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

27877 promoter

Use

Synthetic Biology

Mutation

Promoter sequence mutated to remove sapI sites fo…

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-epyC

Plasmid

#202185

Purpose

Level 0 plasmid containing a terminator sequence from gene 55209 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

epyC terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-acc2

Plasmid

#202184

Purpose

Level 0 plasmid containing a terminator sequence from gene 41316 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

acc2 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-dph1

Plasmid

#202182

Purpose

Level 0 plasmid containing a terminator sequence from gene 54330 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

dph1 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-NR

Plasmid

#202179

Purpose

Level 0 plasmid containing a terminator sequence from gene 54983 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_EF-fcpB

Plasmid

#202177

Purpose

Level 0 plasmid containing a terminator sequence from gene 25172 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

fcpB terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge