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pL0_AC-47740

Plasmid

#202106

Purpose

Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

47740 promoter

Use

Synthetic Biology

Mutation

Promoter sequence mutated to remove bsaI site for…

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-BCA

Plasmid

#202105

Purpose

Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

BCA promoter

Use

Synthetic Biology

Mutation

Promoter sequence mutated to remove sapI sites fo…

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-epyC

Plasmid

#202104

Purpose

Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

epyC promoter

Use

Synthetic Biology

Mutation

Promoter sequence mutated to remove bsaI sites fo…

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-OEE3

Plasmid

#202103

Purpose

Level 0 plasmid containing the promoter from gene 54499 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

OEE3 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-P49

Plasmid

#202102

Purpose

Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-NR

Plasmid

#202101

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-H4

Plasmid

#202100

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_e-4

Plasmid

#202099

Purpose

Receiver cloning vector for even-4 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_e-3

Plasmid

#202098

Purpose

Receiver cloning vector for even-3 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-hSynapsin-Nluc-EPAC-VV

Plasmid

#204846

Purpose

Produce lentivirus encoding Nluc-EPAC sensor in neuronal cell type

Depositor

Mikel Garcia-Marcos

Publication

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

Nluc-EPAC sensor

Use

Lentiviral

Available since

Aug. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_ProRS_TP_GFP_pK7FWG2

Plasmid

#202651

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic ProRS

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_ThrRS_TP_GFP_pK7FWG2

Plasmid

#202652

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic ThrRS

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Shim_TrpRS_TP_GFP_pK7FWG2

Plasmid

#202653

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic TrpRS

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Silene_conica _GlnRS_MitoTP_pK7FWG2

Plasmid

#202654

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic GlnRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Silene_conica _LysRS_MitoTP_pK7FWG2

Plasmid

#202655

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic LysRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Agrostemma_githago _LysRS_MitoTP_pK7FWG2

Plasmid

#202656

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Agrostemma githago cytosolic LysRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Silene_conica _TyrRS_MitoTP_pK7FWG2

Plasmid

#202657

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Silene_vulgaris _TyrRS_MitoTP_pK7FWG2

Plasmid

#202658

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Silene_conica _PheRS_MitoTP_pK7FWG2

Plasmid

#202659

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to mito

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Silene_conica _PheRS_PlastidTP_pK7FWG2

Plasmid

#202660

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Publication

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid

Tags

Green Florescent Protien

Expression

Plant

Available since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge