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pDONR221-SLC25A37_STOP

Plasmid

#161207

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A37 (SLC25A37 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC39A9

Plasmid

#132189

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A9 (SLC39A9 Human)

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC4A10_STOP

Plasmid

#161474

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A10 (SLC4A10 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Ubiquitin K63C

Plasmid

#86589

Purpose

Bacterial expression of Ubiquitin K63C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K63C

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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ACE2(-252)-luc

Plasmid

#31111

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -252 to +103

Available Since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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CD Ugi4

Plasmid

#177196

Purpose

Greengate entry vector pGGC000 modified to contain bladderwort insulator 4

Depositor

Wayne Parrott

Article

Park et al bioRxiv 2021.11.04.467280

Insert

Utricularia gibba insulator 4

Expression

Bacterial and Plant

Available Since

June 28, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-Leum

Plasmid

#44316

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pET28GST-LIC

Plasmid

#26101

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6XHis, GST, and Thrombin CLeavage Site

Expression

Bacterial

Available Since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC22A1

Plasmid

#132034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A1 (SLC22A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-H2B-6

Plasmid

#57384

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H2B (H2BC11 Human)

Tags

mEos2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC10A1_STOP

Plasmid

#161073

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC10A1 (SLC10A1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3DAX

Plasmid

#51333

Depositor

Cheryl Arrowsmith

Insert

3DAX

Expression

Bacterial

Mutation

Truncation: See Comments

Promoter

T7

Available Since

Feb. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4046979

Plasmid

#145611

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1847_2350

Plasmid

#40533

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1847_2350

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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HUNK_pCSdest

Plasmid

#53809

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

HUNK (HUNK Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pDONR221-SLC27A4_STOP

Plasmid

#161449

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC27A4 (SLC27A4 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1932

Plasmid

#79781

Purpose

High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple259

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple259 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEutRwc

Plasmid

#216334

Purpose

An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

Depositor

Mark Styczynski

Article

McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

Inserts

EutR
Superfolder green fluorescent protein

Expression

Bacterial

Available Since

Dec. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-i3 G183S ONE-GO

Plasmid

#204338

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3 G183S ONE-GO

Use

Lentiviral

Mutation

G183S mutation in Galpha-i3 (renders it RGS insen…

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_MFSD10

Plasmid

#131890

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD10 (MFSD10 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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