We narrowed to 3,764 results for: golden gate

1. pGoldenGreenGate-M (pGGG-M)

   Plasmid

   #165422

   Purpose

   A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Level 2 golden gate (moclo) du…

   Promoter

   Contain GG cassette and MCS with LacZ for blue/wh…

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGoldenGate-SE9

   Plasmid

   #47677

   Purpose

   Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and Kanamycin selection.

   Depositor

   Jose Dinneny

   Article

   Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

   Type

   Empty backbone

   Use

   Arabidopsis transformation

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGoldenGate-MCY2

   Plasmid

   #47679

   Purpose

   Binary vector for Arabidopsis transformation with SapI Golden Gate sites and mCherry seed selection

   Depositor

   Jose Dinneny

   Article

   Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

   Type

   Empty backbone

   Use

   Arabidopsis transformation

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGoldenGate-SE7

   Plasmid

   #47676

   Purpose

   Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and mCherry seed selection.

   Depositor

   Jose Dinneny

   Article

   Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

   Type

   Empty backbone

   Use

   Arabidopsis transformation

   Available Since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-GoldenGate

   Plasmid

   #47388

   Purpose

   Expression plasmid for TALE proteins compatible with GoldenGate cloning system

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-GoldenGate-VP64

   Plasmid

   #47389

   Purpose

   Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   FLAG, HA, and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPD005 pcDNA Golden Gate

   Plasmid

   #138749

   Purpose

   Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Golden-Gate-Esp3I-Destination

   Plasmid

   #51278

   Purpose

   CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. BsaI-MCS-cloning vector for golden gate

   Plasmid

   #241214

   Depositor

   Junsu Kang

   Type

   Empty backbone

   Use

   Minicircle cloning vector

   Mutation

   TCGC linker

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

    Plasmid

    #172506

    Purpose

    Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 bacterial expression vector with golden gate site for guides
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

    Plasmid

    #172514

    Purpose

    Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
    

    Use

    AAV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

    Plasmid

    #172508

    Purpose

    Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM3110

    Plasmid

    #40247

    Depositor

    Susan Golden

    Article

    Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13

    Insert

    attP Site
    

    Use

    Cyanobacteria cloning vector

    Promoter

    n/a

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGolden-AAV

    Plasmid

    #51424

    Purpose

    Contain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    lacZ
    

    Use

    AAV

    Promoter

    lacZ

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGolden-Neo

    Plasmid

    #51422

    Purpose

    Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGolden-Hyg

    Plasmid

    #51423

    Purpose

    Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    PGK-Hyg-pA (PGK1 Human)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Gate2 ccdB

    Plasmid

    #117116

    Purpose

    Destination vector

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Type

    Empty backbone

    Use

    Backbone for golden gate assembly for gene target…

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-Gate2 mazEF

    Plasmid

    #117117

    Purpose

    Destination vector with mazEF toxin antitoxin system

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Type

    Empty backbone

    Use

    Backbone for golden gate assembly for gene target…

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-module1

    Plasmid

    #222838

    Purpose

    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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