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p36-p37-p38-p40-pET-Duet-1

Plasmid

#175049

Purpose

Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

Plasmid

#175051

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)

Use

Tags

Expression

Bacterial

Mutation

changed Thr76 to Asp, phosphomimetic mutation

Promoter

T7 promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

Plasmid

#175050

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX

Plasmid

#214284

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Inserts

Split-KRas fragments fused with CIDs and FPs (KRAS Human)
Split-KRas fragments fused with CIDs and FPs (KRAS Human)

Use

Tags

mCerulean (on insert 1) and mVenus (on insert 2)

Expression

Mammalian

Mutation

KRas-Q61L

Promoter

CMV

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX

Plasmid

#214285

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Inserts

Split-KRas fragments fused with CIDs and FPs (KRAS Human)
Split-KRas fragments fused with CIDs and FPs (KRAS Human)

Use

Tags

mCerulean (N terminal on insert 1) and mVenus (on…

Expression

Mammalian

Mutation

KRas-S17N

Promoter

CMV

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

Plasmid

#214286

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Inserts

Split-KRas fragments fused with CIDs and FPs (KRAS Human)
Split-KRas fragments fused with CIDs and FPs (KRAS Human)

Use

Tags

mCerulean (N terminal on insert 1) and mVenus (on…

Expression

Mammalian

Mutation

KRas-Q61L

Promoter

CMV

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX

Plasmid

#214287

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Inserts

Split-KRas fragments fused with CIDs and FPs (KRAS Human)
Split-KRas fragments fused with CIDs and FPs (KRAS Human)

Use

Tags

mCerulean (on insert 1) and mVenus (on insert 2)

Expression

Mammalian

Mutation

KRas-S17N

Promoter

CMV

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2 you

Plasmid

#11084

Depositor

William Talbot

Article

Woods et al PLoS Biol. 2005 Mar . 3(3):e66.

Insert

you (scube2 Zebrafish)

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2005

Availability

Academic Institutions and Nonprofits only
GGDest1-Amp

Plasmid

#157649

Purpose

Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-T ZfHoxb10a

Plasmid

#27530

Depositor

Victoria Prince

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hoxb10a (hoxb10a Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only