Skip to main content
Addgene
Showing: 181 - 200 of 433 results

  2. SaDHFR-pNIC-Bsa4

    Plasmid
    #231039
    Purpose
    Expresses Staphylococcus aureus Dihydrofolate reductase under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    SaDHFR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231039-plasmid-map-sequence-id-462635

    Enlarge

  12. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Article
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Use
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

    Enlarge

  16. pBMN DHFR-HiBiT / Lyt2

    Plasmid
    #179731
    Purpose
    Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites
    Depositor
    James Inglese
    Article
    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23
    Insert
    human DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
    Use
    Retroviral
    Tags
    HiBiT
    Expression
    Mutation
    Promoter
    LTR/MMLV
    Available since
    Oct. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    179731-plasmid-map-sequence-id-361671

    Enlarge

  18. KK601: pMVP (L3-L2) ecDHFR DD + polyA

    Plasmid
    #121790
    Purpose
    pMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    ecDHFR Degron Domain + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121790-plasmid-map-sequence-id-237291

    Enlarge

Showing: 181 - 200 of 433 results