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Plasmid#198856PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND4-W24-L18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro
Plasmid#198844PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertCOX2-Q6-L18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro
Plasmid#198852PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND2-W30-L16 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro
Plasmid#198848PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCYTB-W77-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro
Plasmid#198842PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCOX1-W25-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro
Plasmid#198860PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND6-W82-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87829PurposeHigh-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsMTS
NLS
PTS1
UseTagsExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-miR-Report + MT miR-181 BS in GATA6 3UTR
Plasmid#22795DepositorInsertMutant miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
p-miR-Report + MT miR-181 BS in NLK 3UTR
Plasmid#22791DepositorInsertMutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
p-miR-Report + MT miR-181 BS in CDX2 3UTR
Plasmid#22793DepositorInsertMutant miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-FZY2-S100N-T26I&T77I-UGI-Puro
Plasmid#209645PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-FZY2-S100N-T26I&T77I
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT1H
Plasmid#105706PurposeBacterial expression plasmids for production of recombinant metallothionein 1hDepositorInsertMetallothionein 1h (MT1H Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF
Plasmid#100280PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
UseTagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCAGGS-PHFF-sh-hBVRA
Plasmid#100285PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for human BVRA
UseTagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF-sh-mBVRA
Plasmid#100286PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for mouse BVRA
UseTagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2x-MT2
Plasmid#32102DepositorInsertMT (2x) (Mt1 Mouse)
UseTagsMBPExpressionBacterialMutationPromoterP-lacAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-Mart1-SABR
Plasmid#119052Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBIDepositorInsertA0201-Mart1-SABR
UseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-SABR-Backbone
Plasmid#119050Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only