We narrowed to 207 results for: nano bret
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Plasmid#207149PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ1-LSS-mKATE2(N)
Plasmid#207159PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNADepositorInsertGNG1
ExpressionMammalianMutationN-terminally tagged with LSS-mKATE2Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ2-LSS-mKATE2(N)
Plasmid#207160PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNADepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-dSpCas9
Plasmid#238946PurposeN-terminal fusion of HaloTag to dSpCas9, mammalian expressionDepositorInsertHaloTag
TagsdSpCas9ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαq-124-NLuc
Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsS-246-Nluc
Plasmid#207157PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsL-260-NLuc
Plasmid#207158PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα13-125-NLuc
Plasmid#207155PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα12-133-NLuc
Plasmid#207156PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
nLuc-ΔECD-PTHR1
Plasmid#190011PurposeExpresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domainDepositorInsertnLuc-ΔECD-PTHR1 (PTH1R Human)
ExpressionMammalianAvailable SinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rluc8-NbV5
Plasmid#201479PurposeBRET detection of V5-tagged proteinDepositorInsertNbV5-Rluc8
TagsRluc8ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
G12-CASE
Plasmid#190714PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagsNluc is inserted at A133/F134 within GNA12 and cp…ExpressionMammalianAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
NbV5-Rluc8
Plasmid#201480PurposeBRET detection of V5-tagged proteinDepositorInsertRluc8-NbV5
TagsRluc8ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
L2125-FRB-GPA-NLuc-CyOFP1
Plasmid#222885PurposePositive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.DepositorInsertFRB-GPA-20GS-NanoLuc-10GS-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-NL
Plasmid#113445PurposeProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)DepositorInsertProteinA-NanoLuc
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-NL
Plasmid#113453PurposeLentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)DepositorInsertProteinA-NanoLuc
UseLentiviral and LuciferaseExpressionMammalianPromoterhUbCAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
L2117-FKBP-GPA-114-RH-CyOFP1
Plasmid#222884PurposeBiosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.DepositorInsertFKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only