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We narrowed to 207 results for: nano bret

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Showing: 181 - 200 of 207 results

  1. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid
    #207148
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI1 (GNAI1 Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)K
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207148-plasmid-map-sequence-id-410277

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  2. pcDNA3.1(+)Gαi2-91-NLuc

    Plasmid
    #207149
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI2 (GNAI2 Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)Q
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207149-plasmid-map-sequence-id-410273

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  4. pcDNA3.1(+)GαoA-91-NLuc

    Plasmid
    #207151
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAO
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207151-plasmid-map-sequence-id-410283

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  5. pcDNA3.1(+)GαoB-91-NLuc

    Plasmid
    #207152
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAO
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207152-plasmid-map-sequence-id-410279

    Enlarge

  6. pcDNA3.1(+)Gγ1-LSS-mKATE2(N)

    Plasmid
    #207159
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNA
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNG1
    Expression
    Mammalian
    Mutation
    N-terminally tagged with LSS-mKATE2
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207159-plasmid-map-sequence-id-410272

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  7. pcDNA3.1(+)Gαi3-91-NLuc

    Plasmid
    #207150
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI3 (GNAI3 Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)K
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207150-plasmid-map-sequence-id-410284

    Enlarge

  8. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

    Plasmid
    #207160
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNG2 (GNG2 Human)
    Expression
    Mammalian
    Mutation
    N-terminally tagged with LSS-mKATE2
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207160-plasmid-map-sequence-id-410278

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  9. pcDNA3.1(+)Gαq-124-NLuc

    Plasmid
    #207153
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAQ (GNAQ Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position F(124)E
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207153-plasmid-map-sequence-id-410285

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  10. pcDNA3.1(+)GαsL-260-NLuc

    Plasmid
    #207158
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAS (GNAS Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(260)N
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207158-plasmid-map-sequence-id-410280

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  11. nLuc-ΔECD-PTHR1

    Plasmid
    #190011
    Purpose
    Expresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domain
    Depositor
    Sam Gellman
    Publication
    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nLuc-ΔECD-PTHR1 (PTH1R Human)
    Expression
    Mammalian
    Available since
    Nov. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    190011-plasmid-map-sequence-id-379950

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  12. pcDNA3.1(+)Gα13-125-NLuc

    Plasmid
    #207155
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNA13 (GNA13 Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position F(125)D
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207155-plasmid-map-sequence-id-410275

    Enlarge

  13. pcDNA3.1(+)Gα12-133-NLuc

    Plasmid
    #207156
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNA12 (GNA12 Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position A(133)F
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207156-plasmid-map-sequence-id-410281

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  15. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

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  17. L2125-FRB-GPA-NLuc-CyOFP1

    Plasmid
    #222885
    Purpose
    Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB-GPA-20GS-NanoLuc-10GS-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222885-plasmid-map-sequence-id-440204

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  20. L2117-FKBP-GPA-114-RH-CyOFP1

    Plasmid
    #222884
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222884-plasmid-map-sequence-id-444499

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Showing: 181 - 200 of 207 results