Addgene

We narrowed to 21,474 results for: ESC;

Showing: 1981 - 2000 of 21474 results

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ReNL-Actin_pcDNA3

Plasmid

#89531

Purpose

Red color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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tubulin-OeNL_pcDNA3

Plasmid

#89523

Purpose

Orange color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMB-TGNVHAr

Plasmid

#61179

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-GAr

Plasmid

#61173

Purpose

Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

GmMAN49-mCherry

Use

Tags

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

April 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-MITr

Plasmid

#61199

Purpose

Fluorescent reporter for motochondria ScCOX4sp-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TGN41r

Plasmid

#61177

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-LEr

Plasmid

#61186

Purpose

Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB5A2

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pME18S-12053

Plasmid

#55603

Purpose

Aedes aegypti NPY-like receptor AAEL012053 (NPYLR3) full-length clone in mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pME18S-NPYLR1B-7924A

Plasmid

#52386

Purpose

Aedes aegypti NPY-like receptor AAE007924A (NPYLR1B) full-length clone in mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR1B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pME18S-NPYLR1C-7924B

Plasmid

#52385

Purpose

Aedes aegypti NPY-like receptor AAE007924B (NPYLR1C) full-length clone in mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR1C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pmScarlet-I3_C1

Plasmid

#189756

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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tFucci(CA)5

Plasmid

#153521

Purpose

New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

Depositor

Atsushi Miyawaki

Article

Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

Insert

tFucci(CA)5.

Use

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pH2B-Electra2

Plasmid

#179479

Purpose

Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

H2B-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRFP720-N1

Plasmid

#136560

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP720

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSIJ8

Plasmid

#68122

Purpose

Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Inserts

flp recombinase
rhaS
rhaR

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhamnose

Available since

Dec. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-mRuby2-Gephyrin.FingR-IL2RGTC

Plasmid

#125696

Purpose

Global labeling of inhibitory synapses (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mRuby2-Gephyrin.FingR-IL2RGTC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFV2-HA

Plasmid

#187082

Purpose

Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

Depositor

Steven Vik

Article

Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

Insert

atpBEFHAGDC

Use

Tags

6-His beta subunit (atpD) and HA tag subunit a (a…

Expression

Bacterial

Mutation

All Cys codons changed to Ala.

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet3_C1

Plasmid

#189753

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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p.UTA.3.0 Empty

Plasmid

#82447

Purpose

[Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Type

Empty backbone

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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