We narrowed to 7,572 results for: Lif

1. HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw

   Plasmid

   #162471

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreB
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SMT215

   Plasmid

   #134818

   Purpose

   SMT205 with DHFR-FLAG-tag, western blot

   Depositor

   Tanja Kortemme

   Article

   Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

   Inserts

   Dihydrofolate reductase
   Thymidylate synthase
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Silent mutations to eliminate internal restrictio…

   Promoter

   T7 and TET

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SMT101

   Plasmid

   #134815

   Purpose

   Dual expression of DHFR and TYMS, in vivo assays

   Depositor

   Tanja Kortemme

   Article

   Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

   Inserts

   Dihydrofolate reductase
   Thymidylate synthase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutations to eliminate internal restrictio…

   Promoter

   T7

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SMT201

   Plasmid

   #134816

   Purpose

   SMT101 with TET promoter for TYMS, in vivo assays

   Depositor

   Tanja Kortemme

   Article

   Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

   Inserts

   Dihydrofolate reductase
   Thymidylate synthase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutations to eliminate internal restrictio…

   Promoter

   T7 and TET

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGI3EM29

   Plasmid

   #135489

   Purpose

   Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr hph-mCitrine
   

   Use

   Tags

   mCitrine

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGI3EM30

   Plasmid

   #135490

   Purpose

   Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr hph-mClover3
   

   Use

   Tags

   mClover3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGI3EM31

   Plasmid

   #135491

   Purpose

   Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr hph-mCerulean3
   

   Use

   Tags

   mCerulean3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FoxFTVC+bpFOG_MEK-S216D, S220E

   Plasmid

   #107522

   Purpose

   Constitutive activation of FGF/MAPK pathway by MEK-S216D, S220E mutant

   Depositor

   Lionel Christiaen

   Article

   Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

   Insert

   MEK
   

   Use

   Tags

   Expression

   Mutation

   changed Serine 216 to Aspartic Acid and Serine 22…

   Promoter

   TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PTEN-3'UTR

   Plasmid

   #97204

   Purpose

   Expresses PTEN 3'UTR in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Kerwin et al Elife. 2020 Apr 21;9. pii: 51019. doi: 1

   Insert

   PTEN 3' UTR (PTEN Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Thy1.1-muSOX D219A

    Plasmid

    #131704

    Purpose

    encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-muSOX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Aspartic acid 219 mutated to an alanine

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k

    Plasmid

    #127403

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

    Plasmid

    #127407

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, K305H,…

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

    Plasmid

    #127401

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXW109ABS62(RS)

    Plasmid

    #123153

    Purpose

    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS62, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXW109ABS67(RS)

    Plasmid

    #123154

    Purpose

    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109. ParsR-ABS67, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P-1 gnu

    Plasmid

    #112999

    Purpose

    For protein expression and purification of full-length Drosophila gnu

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length gnu (gnu Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-SAC1C389S-HLx8

    Plasmid

    #108140

    Purpose

    Inactive SAC1 with extended linker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRG312-slc1a-5

    Plasmid

    #99102

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea slc1a-5 (also known as excitatory amino acid transporter)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

    Insert

    slc1a-5 (also known as excitatory amino acid transporter) in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRG94-runt-1

    Plasmid

    #99089

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Runt-1 (also known as runt)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

    Insert

    Runt-1 (also known as runt) in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3.2mar(T->G)-Luc

    Plasmid

    #63697

    Purpose

    3.2 kb marine armor plate enhancer with a single T->G bp change driving Luciferase expression

    Depositor

    David Kingsley

    Article

    O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

    Insert

    3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    TATA box

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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