We narrowed to 2,128 results for: Pam

1. pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

   Plasmid

   #185328

   Purpose

   For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   MAHS_RAMVA_81-236-FLAG
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR V2 sgYME1L1 sg1

   Plasmid

   #244867

   Purpose

   Knockout of human YME1L1

   Depositor

   Alexis Jourdain

   Article

   Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

   Insert

   YME1L1 sgRNA (YME1L1 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Nov. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPR V2 sgYME1L1 sg2

   Plasmid

   #244868

   Purpose

   Knockout of human YME1L1

   Depositor

   Alexis Jourdain

   Article

   Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

   Insert

   YME1L1 sgRNA (YME1L1 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. EW488 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(T2098L)

   Plasmid

   #236159

   Purpose

   Express NZF transcriptional activation domain fused to a deimmunized linker and mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   NZF-deImmunLink-FRB
   

   Expression

   Mammalian

   Mutation

   T2098L in FRB

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. L2117-FKBP-GPA-114-RH-CyOFP1

   Plasmid

   #222884

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. L2116-FRB-GPA-114-RH-CyOFP1

   Plasmid

   #222883

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114-RH-CyOFP1
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. L2379-FKBP-GPA(V84R)-20-11S

   Plasmid

   #222901

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA(V84R)-20GS-NanoLuc11S
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. L2380-FKBP-GPA(V84R)-20-114

   Plasmid

   #222900

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA(V84R)-20GS-NanoLuc114
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. L2378-FRB-GPA(V84R)-20-11S

   Plasmid

   #222899

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(V84R)-20GS-NanoLuc11S
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L2377-FRB-GPA(V84R)-20-114

    Plasmid

    #222898

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-20GS-NanoLuc114
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

    Plasmid

    #222875

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. L2132-FKBP-GPA-114-5-CyOFP1

    Plasmid

    #222891

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. L2127-FRB-GPA-114-20-CyOFP1

    Plasmid

    #222886

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L2129-FRB-GPA-114-5-CyOFP1

    Plasmid

    #222888

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L2128-FRB-GPA-114-10-CyOFP1

    Plasmid

    #222887

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L2131-FKBP-GPA-114-10-CyOFP1

    Plasmid

    #222890

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L2130-FKBP-GPA-114-20-CyOFP1

    Plasmid

    #222889

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-FKBP-LBR

    Plasmid

    #222427

    Purpose

    To express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR (LBR Human)
    

    Tags

    GFP-FKBP

    Expression

    Mammalian

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT309 RapInducible1-ActivatorCloneSite-Citrine

    Plasmid

    #187321

    Purpose

    Rapamycin inducible genetic circuit with cloning site for activator domains

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. DRD4-DuET

    Plasmid

    #213230

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    DRD4 (DRD4 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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