We narrowed to 14,886 results for: pET

1. p3990 pET ubcH8

   Plasmid

   #8650

   Depositor

   Peter Howley

   Article

   Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

   Insert

   UbcH8 (UBE2L6 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pET28_human-pro-IL-18_DeltaN24

   Plasmid

   #214327

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Deletion of first 24 amino acids

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28_Caspase-5-enzymatic-domain

   Plasmid

   #214306

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP5 (CASP5 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   aa133-434

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15b-17X-PTDH

   Plasmid

   #166786

   Purpose

   Phosphite dehydrogenase for NAD(P)H regeneration

   Depositor

   Wilfred van der Donk

   Article

   Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

   Insert

   Phosphite dehydroenase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   A196R, T201S, A328T, E352N, and C356D- please see…

   Promoter

   T7

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21-pelB-LaG-18

   Plasmid

   #172763

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-18
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET15b LFn-Fla

   Plasmid

   #84871

   Purpose

   Bacterial expression of LFn-Fla fusion

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Fla
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET28b-CoilR-Cys

   Plasmid

   #181989

   Purpose

   Used to label proteins with the CoilE genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

   Insert

   CoilR-Cys
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLicC-MBP-APETx2

   Plasmid

   #72668

   Purpose

   Periplasmic expression of APETx2 in E.coli

   Depositor

   Glenn King

   Article

   Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

   Insert

   APETx2
   

   Use

   Tags

   His tag, MBP, MalE signal sequence for periplasmi…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28-Mff(1-61)-PP-GST

   Plasmid

   #73042

   Purpose

   Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

   Depositor

   David Chan

   Article

   Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

   Insert

   Mff(1-61) (Mff Mouse)
   

   Use

   Tags

   GST-tag and PreScission protease site

   Expression

   Bacterial

   Mutation

   Truncation containing amino acids 1-61

   Promoter

   T7

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaG-10

    Plasmid

    #172743

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-10
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET23a-His-hRanGAP1tail

    Plasmid

    #53139

    Purpose

    E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    RanGAP1 (RANGAP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C-terminal fragment (amino acids 398-587)

    Promoter

    T7

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaG-42

    Plasmid

    #172758

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-42
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-45b-mEGFP

    Plasmid

    #185013

    Purpose

    Bacterial Expression of 6xHis Sirius-GFP Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    Sirius-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-45b-mCherry

    Plasmid

    #145279

    Purpose

    Bacterial Expression of 6xHis mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-PTBP1-RRM34

    Plasmid

    #89156

    Purpose

    Recombinant expression of PTBP1-RRM34 in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    contains RNA Recognition Motif 3+4

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET26-MBP-GLIC

    Plasmid

    #20887

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421
    

    Use

    Tags

    His, MBP, and pelB

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-51b-HaloTag11

    Plasmid

    #175519

    Purpose

    Expression of HaloTag11 in bacterial cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag11
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET3-ARL4C-His6

    Plasmid

    #201869

    Purpose

    Used for bacterial expression of human protein ARL4C

    Depositor

    Richard Kahn

    Insert

    ARL4C (ARL4C Human)
    

    Use

    Tags

    6xHis (GSGSGSHHHHHH)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-TEV-U1AF37MF77M

    Plasmid

    #168267

    Purpose

    Expresses human dmU1A with the F37M/F77M double mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    U1A RRM1 crystallization module (SNRPA Human)
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F37M and F77M

    Promoter

    T7 promotor

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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