We narrowed to 5,756 results for: cki

1. pET21b-LaG9(S151C)

   Plasmid

   #172490

   Purpose

   Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   LaG9(S151C)
   

   Use

   Tags

   6xHis, PPX cleavage site, and pelB leader sequenc…

   Expression

   Bacterial

   Mutation

   S151C

   Promoter

   T7

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AX53_pS.Donor.S1.TS

   Plasmid

   #100290

   Purpose

   AAVS1-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   18 bp RFLP (MluI+KspAI)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mini-donor - NKX2.5-nEGFP

   Plasmid

   #209287

   Purpose

   this mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locus

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

   Insert

   EGFP-nls
   

   Use

   Knockin donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58

   Plasmid

   #171432

   Purpose

   Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-hH3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDH42(CUG/A)

   Plasmid

   #154132

   Purpose

   Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   engineered bacterial rRNAs operon under control of pL promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-MEIOC

   Plasmid

   #222562

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   MEIOC (C17orf104 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-ERK2 D319N

   Plasmid

   #8979

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D319N

   Promoter

   Available since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

8. Fast-GCaMP-EF20

   Plasmid

   #52645

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF20
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D362N/D366N mutated from GCaMP3

   Promoter

   T7

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

9. SLC30A10-GFP

   Plasmid

   #104381

   Purpose

   Expresses SLC30A10-GFP wild-type in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

   Insert

   SLC30A10 (SLC30A10 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. YFP-mDia1FH2deltaN

    Plasmid

    #25419

    Depositor

    Arthur Alberts

    Article

    Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

    Insert

    diaph1 (Diaph1 Mouse)
    

    Use

    Tags

    YFP A206K

    Expression

    Mammalian

    Mutation

    mDia1 FH2∆N2 sequence (encodes amino acids 771-11…

    Promoter

    Available since

    July 20, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

    Plasmid

    #171427

    Purpose

    Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CMV-NLS-dCas9-NLS

    Plasmid

    #99908

    Purpose

    For the expression of NLS-dCas9-NLS

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Insert

    NLS-dCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. UNC50-myc

    Plasmid

    #104382

    Purpose

    Expresses UNC50-myc wild-type in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

    Insert

    UNC50 (UNC50 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-cT3G-cERP2-DMC1

    Plasmid

    #222541

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of DMC1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    DMC1 (DMC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. dABE

    Plasmid

    #124447

    Purpose

    Expresses dead Cas9 (dCas9)-ABE7.10

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dABE7.10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid

    #74709

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-IREShygro2

    Plasmid

    #153966

    Purpose

    Expresses EGFP in mammalian cells

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    EGFP
    

    Use

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    No mutation

    Promoter

    CMV promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1α-squirrel-monkey-retroCHMP3-HA

    Plasmid

    #154174

    Purpose

    expresses squirrel monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AV09_pDonor.EP.S1.TS

    Plasmid

    #100297

    Purpose

    AAVS1-targeting expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    PuroR.T2A.EGFP expression unit
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Fast-GCaMP-EF16

    Plasmid

    #52642

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D431N/D433N/D435N/E442A mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only