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Viral Prep#105669-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsFusionRedAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAAV-CKIIa-stGtACR2-FusionRed (AAV Retrograde)
Viral Prep#105669-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsFusionRedAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
eDrcKIT
Plasmid#184001PurposeOptically controlled eDrcKIT expression in mammalian cellsDepositorInserteDrcKIT (KIT Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCKIPSD-1/1
Plasmid#91376PurposeProtein expression and purification of human SH3 domain construct NCKIPSD-1/1DepositorInsertNCKIPSD-1/1 (NCKIPSD Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgCTNNB1 Nicking
Plasmid#169845PurposeExpress a nicking sgRNA used for installation of the oncogenic S45F or S45del in Ctnnb1 in mouse liver.DepositorInsertsgCTNNB1 Nicking (Ctnnb1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDOCKING-Neo
Plasmid#31441DepositorInsertECFP-neomycin
UseCre/Lox; Phic31/attpTagsECFPExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
sgAAT Nicking
Plasmid#169842PurposeExpress a nicking sgRNA used for correction (via A•T-to-G•C) of the E342K mutationDepositorInsertsgAAT Nicking (SERPINA1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 4
Plasmid#99319PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
V405 4HA-CKIe
Plasmid#13724DepositorInsertcasein kinase 1 epsilon (CSNK1E Human)
UseTags4 HAExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-cKit
Plasmid#114293PurposeTET-inducible expression of c-KitDepositorInsertcKit (Kit Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CKI(0.4)_HA_AzPPO_AgeI_nrxn1a_WT_IRES_mScarlet
Plasmid#205289PurposeBistable opsin for neuronal inhibitionDepositorInsertParapinopsin
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 3
Plasmid#99318PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Miro1 (lacking TM)
Plasmid#127613PurposeHuman Miro1 with the transmembrane deletedDepositorInsertMiro1 (RHOT1 Human)
UseTagsmycExpressionMammalianMutationDeletion of transmembrane domainPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 1
Plasmid#99316PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 1
Plasmid#207607PurposesgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertcgactcgcccggcagcgcac
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 2
Plasmid#207608PurposesgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertacccccaaacctgactgact
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CKII(0.4)_AzPPO_D14_ts_mScarlet_Kv2.1
Plasmid#205288PurposeBistable opsin for neuronal inhibitionDepositorInsertParapinopsin
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
myc-Miro2 (lacking TM)
Plasmid#127614PurposeHuman Miro2 with the transmembrane deletedDepositorInsertMiro2 (RHOT2 Human)
UseTagsmycExpressionMammalianMutationTransmembrane deletionPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only