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pET28CLY6

Plasmid

#21765

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pET28CLY4

Plasmid

#21763

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 4 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pET28CLY8

Plasmid

#21767

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pSG5-myc-p110α-CAAX

Plasmid

#39494

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Use

Eukaryotic

Tags

CAAX and myc

Expression

Mutation

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-myc-p110α(K227E)

Plasmid

#39495

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110(K227E) (PIK3CA Bovine)

Use

Eukaryotic

Tags

myc

Expression

Mutation

K227E

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pAAV2-CAGIG-WGA-mCherry

Plasmid

#224119

Purpose

To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.

Depositor

Pantelis Tsoulfas

Insert

WGA-mCherry

Use

AAV

Tags

WGA and mCherry

Expression

Mammalian

Mutation

All mutations related to the WGA in this construc…

Promoter

CAGIG

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN10-gCTG

Plasmid

#114385

Purpose

pPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTG

Depositor

Vincent Dion

Article

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gCTG

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYes2-TaPCS1

Plasmid

#49767

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Article

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

TaPCS1 (PCS1 Triticum aestivum)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pPN10-gCAG

Plasmid

#114384

Purpose

pPN10 with gRNA for spCRISPR-Cas9 targeted to CAG repeat, PAM: CAG

Depositor

Vincent Dion

Article

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gCAG

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc-p110α-CAAX

Plasmid

#39490

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Use

Tags

CAAX and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc-p110α_wt

Plasmid

#39489

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
pGADT7-RWT4Ca

Plasmid

#237524

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN10-gDM1d

Plasmid

#114387

Purpose

pPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGG

Depositor

Vincent Dion

Article

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gDM1d

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3TS-Tol2

Plasmid

#31831

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Lac

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/shp53/GFP4

Plasmid

#20208

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

shp53/GFP4

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Guillaume Cambray, Adam Arkin

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pbad and Ptet

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2KXIGdeltaIn-MCS-birA-tagRFP

Plasmid

#58378

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA tagRFP

Use

Tags

tagRFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2KXIGdeltaIn-MCS-birA-EGFP

Plasmid

#58377

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA EGFP

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc-p110α(K227E)

Plasmid

#39491

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110(K227E) (PIK3CA Bovine)

Use

Tags

myc

Expression

Mammalian

Mutation

K227E

Promoter

CMV

Available since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only