We narrowed to 5,495 results for: IRES

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AiP1199 - pAAV-AiE2010m-minBG-IRES2-iCre-HGHpA

Plasmid

#230585

Purpose

AiE2010m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre

Use

AAV

Promoter

minBG

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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AiP982 - pAAV-AiE2004m-minBG-IRES2-iCre-HGHpA

Plasmid

#230568

Purpose

AiE2004m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre

Use

AAV

Promoter

minBG

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA

Plasmid

#233040

Purpose

To express mouse homeostatic iron regulator (HFE)(H67D) from an EF1a promoter. It also encodes GFP after an IRES sequence

Depositor

Jonathan Ploski

Insert

mouse homeostatic iron regulator with H67D mutation (Hfe Mouse)

Use

AAV

Tags

GFP

Mutation

H67D

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9

Plasmid

#231909

Purpose

For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1666 - pAAV-AiE0486m-minBG-IRES2-iCre-HGHpA

Plasmid

#220710

Purpose

AiE0486m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

iCre

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

Plasmid

#232246

Purpose

Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Mutation

delta73-82

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

Plasmid

#231233

Purpose

MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Expression

Mammalian

Mutation

TAD deleted (delta1-143); numbering based on sequ…

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcs2-cDELE1(142-515)-GFP-IRES-mCherry

Plasmid

#226102

Purpose

cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

DELE1 (DELE1 Human)

Tags

GFP

Expression

Mammalian

Mutation

only encodes for residues 142-515 of DELE1 corres…

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

Plasmid

#225490

Purpose

Neuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Neuronal expression

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

Plasmid

#224532

Purpose

A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-H2A-mCherry-SV40pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD H1734A H1741A

Plasmid

#169830

Purpose

Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

H1734A H1741A; TCCC -> AGTC silent mutations a…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD delta Loop1

Plasmid

#169831

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids F1366 - P1376; TCCC ->…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD delta Loop2

Plasmid

#169832

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids R1398 - S1407; TCCC ->…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD T456A S1406A

Plasmid

#169823

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1406A; TCCC -> AGTC silent mutations at…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD T456A S1859A

Plasmid

#169824

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1859A; TCCC -> AGTC silent mutations at…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218637

Purpose

Proximity biotinylation control of OSBP(PH domain)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

PH domain only

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

Plasmid

#207171

Purpose

expression plasmid for Gal4DBD-HIF-CAD.myc expression

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD Myc tag linker

Tags

2xMyc tag

Expression

Mammalian

Promoter

EF1a

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

Plasmid

#202090

Purpose

Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2(R195G, C201A)

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

R195G and C201A abolish catalytic activity

Promoter

human Synapsin

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD R1403A R1404A

Plasmid

#188135

Purpose

Expresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD E1367A E1368A

Plasmid

#188143

Purpose

Expresses C-terminal flag-tagged human CAD E1367A E1368A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge