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pRK5_mEGFP-NUP98-DDX10-Mut1

Plasmid

#237647

Purpose

For overexpression of mEGFP-NUP98-DDX10-Mut1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-DDX10-Mut1 (NUP98, DDX10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-DDX10-Mut2

Plasmid

#237648

Purpose

For overexpression of mEGFP-NUP98-DDX10-Mut2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-DDX10-Mut2 (NUP98, DDX10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-DDX10-KS-FtoG

Plasmid

#237645

Purpose

For overexpression of mEGFP-NUP98-DDX10-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-DDX10-KS-FtoG (NUP98, DDX10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-FUS-gRNA

Plasmid

#237685

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to FUS locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

FUS gRNA (FUS Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK12deltaNTD /Cyclin K

Plasmid

#231741

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1490)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK12deltaCTD/Cyclin K

Plasmid

#231742

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(1-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK12-KiD/Cyclin K

Plasmid

#231743

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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MKC106_HBB(HBA1reg-HBA1-IRES-tEPOR)

Plasmid

#232411

Purpose

AAV production plasmid for HBA+tEPOR vector from Figs. 4-6 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA+tEPOR cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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VEGF120TAG

Plasmid

#200946

Purpose

Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF120TAG (Vegfa Mouse)
hPGK-driven Puromycin resistance

Use

Lentiviral

Tags

6x HIS

Mutation

amber stop codon 101 bp from ATG

Promoter

Ef1a and hPGK

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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VEGF164TAG

Plasmid

#200947

Purpose

Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF164TAG (Vegfa Mouse)
hPGK-driven Puromycin resistance

Use

Lentiviral

Tags

6x HIS

Mutation

amber stop codon 101 bp from ATG

Promoter

Ef1a and hPGK

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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