We narrowed to 5,024 results for: PID

1. pMK243 (Tet-OsTIR1-PURO)

   Plasmid

   #72835

   Purpose

   Expresses OsTIR1 under the control of the Tet promoter

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   AAVS1-Tet-OsTIR1-PURO-AAVS1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAD-GamS

   Plasmid

   #99246

   Purpose

   Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   gamS (with C-terminal 6xHis tag)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-FKBP-SAC1deltaTMD

   Plasmid

   #108132

   Purpose

   Recruitable cytosolic SAC1

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SACM1L(1-521)

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CTB

   Plasmid

   #141166

   Purpose

   Expresses recombinant Cholera Toxin B Subunit

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   CTB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No secretion signal

   Promoter

   T7

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDN-Sth1Cas9

   Plasmid

   #221386

   Purpose

   Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   Sth1 Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDN-Cherry-sgRNA

   Plasmid

   #221387

   Purpose

   episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   none, cloning vector for introduction of targeting sequences
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi_RP_MicroID2-HA

   Plasmid

   #188521

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS4333 yeTadA1.0-T7 RNAP

   Plasmid

   #137735

   Purpose

   pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

   Depositor

   Christina Smolke

   Article

   Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

   Insert

   yeTadA1.0-T7 RNAP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   E366G (See depositor comment below)

   Promoter

   Available since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSP1E3D1_D73C

   Plasmid

   #39328

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009 . 464():211-31.

   Insert

   MSP1E3D1_D73C (APOA1 synthetic gene)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   cys mutant of MSP1E3D1

   Promoter

   T7

   Available since

   Sept. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

10. Omega Destination-CMV::ELuc:bGH

    Plasmid

    #124528

    Purpose

    Omega destination vector, that has a CMV:ELUC:bgH in the backbone

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRPML1-YFP

    Plasmid

    #18826

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST14-SpyCatcher

    Plasmid

    #35044

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyCatcher
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Deletion of C-terminal till I113, and point mutat…

    Promoter

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-EGFP-Rac1-Q61L

    Plasmid

    #12981

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDNR-gRNA

    Plasmid

    #206990

    Purpose

    A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Insert

    Donor plasmid-targeting SaCas9-gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-H2B-mEGFP

    Plasmid

    #105528

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    H2B (H2BC11 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-EGFP-Rac1-T17N

    Plasmid

    #12982

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 DN (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Lv EF1a SS18-Frb PGK Puro

    Plasmid

    #102811

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    SS18-Frb
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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