Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,567 results for: puro

Showing: 2021 - 2040 of 18567 results

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CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2

Plasmid

#231207

Purpose

Single transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Publication

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Use

Lentiviral

Mutation

L147I, R148H, I203V, L290V

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-NCOA7g3 (BB24)

Plasmid

#139459

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Publication

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-mir144~451 EF1Alpha-puro-T2A-BFP

Plasmid

#164793

Purpose

Express miR-144~451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144~451 (MIR451A, MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-KIF5AR280H-HA-IRES-Puro

Plasmid

#166953

Purpose

Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A-R280H (Kif5a Mouse)

Use

Lentiviral

Tags

HA

Mutation

KIF5A-R280H

Promoter

eF1a

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN2-C911

Plasmid

#125786

Purpose

a seed-matched control for pRSITEP-puro-shWRN2

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV-puro-N3FLAG-MYB-del(TAD)

Plasmid

#105597

Purpose

retrovirally express mouse MYB-del(TAD) with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Publication

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB without TAD domain (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

deletion of TAD (AA 275-327)

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZNF90_5-5)-PGKpuroBFP-W

Plasmid

#212003

Purpose

Express gRNA against ZNF90 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZNF90 (ZNF90 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(VENTX_5-5)-PGKpuroBFP-W

Plasmid

#211993

Purpose

Express gRNA against VENTX with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting VENTX (VENTX Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZIC2_5-4)-PGKpuroBFP-W

Plasmid

#211994

Purpose

Express gRNA against ZIC2 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZIC2 (ZIC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZIC2_5-5)-PGKpuroBFP-W

Plasmid

#211995

Purpose

Express gRNA against ZIC2 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZIC2 (ZIC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZIC3_5-3)-PGKpuroBFP-W

Plasmid

#211996

Purpose

Express gRNA against ZIC3 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZIC3 (ZIC3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZIC3_5-5)-PGKpuroBFP-W

Plasmid

#211997

Purpose

Express gRNA against ZIC3 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZIC3 (ZIC3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZNF398_5-2)-PGKpuroBFP-W

Plasmid

#211998

Purpose

Express gRNA against ZNF398 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZNF398 (ZNF398 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZNF398_5-5)-PGKpuroBFP-W

Plasmid

#211999

Purpose

Express gRNA against ZNF398 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZNF398 (ZNF398 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZNF649_5-1)-PGKpuroBFP-W

Plasmid

#212000

Purpose

Express gRNA against ZNF649 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZNF649 (ZNF649 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZNF649_5-5)-PGKpuroBFP-W

Plasmid

#212001

Purpose

Express gRNA against ZNF649 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZNF649 (ZNF649 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZNF90_5-3)-PGKpuroBFP-W

Plasmid

#212002

Purpose

Express gRNA against ZNF90 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZNF90 (ZNF90 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(TRIM24_4)-PGKpuroBFP-W

Plasmid

#211988

Purpose

Express gRNA against TRIM24 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting TRIM24 (TRIM24 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(TRIM24_5-4)-PGKpuroBFP-W

Plasmid

#211989

Purpose

Express gRNA against TRIM24 with puro and BFP

Depositor

Kosuke Yusa

Publication

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting TRIM24 (TRIM24 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2021 - 2040 of 18567 results

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