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pHK001.5

Plasmid

#235453

Purpose

NOT gate receiver with bs for sgRNA5

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK001.6

Plasmid

#235454

Purpose

NOT gate receiver with bs for sgRNA6

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-DD-T6B-WT-YFP

Plasmid

#235145

Purpose

Expresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused T6B peptide

Use

AAV

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-FLAG-HA-T6B-WT-YFP

Plasmid

#235141

Purpose

Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

T6B peptide

Use

AAV

Tags

FLAG/HA

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-roxSTOProx-NaChBac-TdTomato

Plasmid

#236253

Purpose

Dre dependent NaChBac-TdTomato expression

Depositor

Janelia Viral Tools

Article

Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

Insert

NaChBac-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Promoter

hSyn

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iC

Plasmid

#231418

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-SynCas.v10 bacteria

Plasmid

#208594

Purpose

Production and purification of catalytically active SynCas.v10

Depositor

Omar Akbari

Article

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

SynCas.v10

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria

Plasmid

#208595

Purpose

Production and purification of catalytically inactive SynCas.v10

Depositor

Omar Akbari

Article

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

dSynCas.v10

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-SynCas.v12 bacteria

Plasmid

#208596

Purpose

Production and purification of catalytically active SynCas.v12

Depositor

Omar Akbari

Article

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

SynCas.v12

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria

Plasmid

#208597

Purpose

Production and purification of catalytically inactive SynCas.v12

Depositor

Omar Akbari

Article

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

dSynCas.v12

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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