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pcDNA3.1 Cas9-P2A-dTomato-T20

Plasmid

#231912

Purpose

For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

Cas9-P2A-dTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter dme-let-7

Plasmid

#46662

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-let-7

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter dme-mir-34

Plasmid

#46665

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-34

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tras heavy chain-GSG-SpyTag003

Plasmid

#216292

Purpose

Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Tras heavy chain-GSG-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1.2

Plasmid

#46681

Purpose

Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1.2 (MIR16-1 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1.3

Plasmid

#46682

Purpose

Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1.3 (MIR16-1 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1.1

Plasmid

#46680

Purpose

Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1.1 (MIR16-1 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

Plasmid

#204694

Purpose

This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT (BRD4, NUTM1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

direct fusion of BRD4 and NUT

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300

Plasmid

#204697

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300 (BRD4, EP300 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

direct fusion of BRD4 to p300

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)

Plasmid

#204700

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300(I) (BRD4, EP300 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of all TF-bidning domains and HAT in p300

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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