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We narrowed to 6,946 results for: pcDNA3.1+

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Showing: 2041 - 2060 of 6946 results

  1. pCDNA3.1-nAChr-beta3

    Plasmid
    #48811
    Purpose
    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.
    Depositor
    Henry Lester, Jerry Stitzel
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    Expression
    Mammalian
    Mutation
    *
    Promoter
    CMV
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    48811-plasmid-map-sequence-id-193963

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  2. pcDNA3.1-Firefly-ccdB

    Plasmid
    #87069
    Purpose
    Mammalian Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87069-plasmid-map-sequence-id-307652

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  5. pcDNA3.1(+)MBII-85-5'/3'ss mut

    Plasmid
    #67645
    Purpose
    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.
    Depositor
    Stefan Stamm
    Publication
    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00
    Insert
    MBII85 snoRNA
    Expression
    Mammalian
    Mutation
    5' and 3' splice sites mutations
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    67645-plasmid-map-sequence-id-116914

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  10. pcDNA3.1/hpa5(aIIB_thigh)

    Plasmid
    #59971
    Purpose
    integrin a5 chimeric headpiece expression (a5 beta-propeller domain and aIIB thigh domain)
    Depositor
    Timothy Springer
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human integrin a5 beta-propeller domain fused with human integrin aIIB thigh domain (ITGA5, ITGA2B Human)
    Tags
    Acid coiled-coil and StrepII tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. TRUPATH Triple Ga13

    Plasmid
    #196062
    Purpose
    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196062-plasmid-map-sequence-id-385881

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  16. eGFP-proα2(I)

    Plasmid
    #119826
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Publication
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    119826-plasmid-map-sequence-id-233864

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  20. Rlucrarr3-Flash6

    Plasmid
    #74134
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.
    Depositor
    Louis Luttrell
    Publication
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 2041 - 2060 of 6946 results