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Showing: 4121 - 4160 of 8321 results

  1. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid
    #72255
    Purpose
    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre-P2A-tdTomato-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  2. pTRIPZ(M)-HT-Cbx8

    Plasmid
    #82516
    Purpose
    Lentivirus vector. Expresses halotag Cbx8fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 8 (CBX8 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82516-plasmid-map-sequence-id-156792

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  4. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-440858

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  5. pcDNA3.1 CLN3-GFP

    Plasmid
    #78110
    Purpose
    Mammalian expression of human CLN3 with C terminal GFP fusion
    Depositor
    Thomas Braulke
    Insert
    CLN3 (CLN3 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. HaloTag-G protein gamma-2

    Plasmid
    #67018
    Purpose
    G protein gamma-2 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-G protein gamma-2 (GNG2 Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67018-plasmid-map-sequence-id-115487

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  7. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

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  9. pENTR1A-Huwe1

    Plasmid
    #37431
    Depositor
    Jean Cook
    Insert
    Huwe1 (HUWE1 Human)
    Use
    Gateway entry
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    37431-plasmid-map-sequence-id-182534

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  10. Pet28a_Synthetic_Human_H2AZ.1

    Plasmid
    #42629
    Depositor
    Joe Landry
    Insert
    Human H2AZ.1 (H2AZ1 Synthetic, Codon Optimized, Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available since
    March 21, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42629-plasmid-map-sequence-id-434040

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  11. pLenti-NPY-mNeonGreen

    Plasmid
    #232456
    Purpose
    Expresses human NPY-mNeonGreen fusion in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    NPY-mNeonGreen (NPY Human)
    Use
    Lentiviral
    Tags
    mNeonGreen
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232456-plasmid-map-sequence-id-461197

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  12. Mek1

    Plasmid
    #40774
    Depositor
    Dustin Maly
    Insert
    MKK1 (MAP2K1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Starting Met removed
    Promoter
    CMV
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only

  13. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid
    #196727
    Purpose
    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    TRE
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196727-plasmid-map-sequence-id-389689

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  14. NPY-GFP

    Plasmid
    #74629
    Purpose
    human neuropeptide Y fused to GFP
    Depositor
    Justin Taraska
    Insert
    Neuropeptide Y (NPY Human)
    Use
    Tags
    mEGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74629-plasmid-map-sequence-id-140841

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  15. pcDNA3.1+ SMN1 myc HIS

    Plasmid
    #71687
    Purpose
    Mammalian expression of SMN1
    Depositor
    Gideon Dreyfuss
    Insert
    SMN1 (SMN1 Human)
    Use
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    71687-plasmid-map-sequence-id-287410

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  16. EF1α-AausFP1-Z1

    Plasmid
    #135614
    Purpose
    AausFP1 - bright green fluorescent protein expression from A. australis under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    AausFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-HTLV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135614-plasmid-map-sequence-id-269404

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  17. pcDNA3.1-Flag-RBM24

    Plasmid
    #208052
    Purpose
    Expressing N-ter Flag tagged human RBM24 (ENST00000379052) in mammalian cells
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208052-plasmid-map-sequence-id-418311

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  18. NET43 pEGFP-N2 (609)

    Plasmid
    #61991
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET43 (NAT10 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211342
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211342-plasmid-map-sequence-id-415648

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  20. pFastBac1_FLAG-BPTF

    Plasmid
    #102242
    Purpose
    Expression of human Flag-BPTF using baculovirus from pFastBac1
    Depositor
    Joe Landry
    Insert
    Flag-BPTF (BPTF Human)
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    Polyhedron
    Available since
    Dec. 11, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102242-plasmid-map-sequence-id-197767

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  21. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    40786-plasmid-map-sequence-id-68901

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  22. pEGFP Cited2

    Plasmid
    #112246
    Purpose
    expression of EGFP-Cited2 in mammalian cells
    Depositor
    Simone Di Giovanni
    Insert
    Cited2 (CITED2 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112246-plasmid-map-sequence-id-213576

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  23. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    40787-plasmid-map-sequence-id-68902

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  24. pLJM1-4EBP1-T37A/T46A

    Plasmid
    #136420
    Purpose
    Stable overexpression of elF4EBP1 T37A/T46A
    Depositor
    Gabriel Leprivier
    Insert
    eIF4EBP1 (EIF4EBP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T37A/T46A
    Promoter
    CMV
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136420-plasmid-map-sequence-id-270114

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  25. Myc-Foxo1 AAA (pCMV5)

    Plasmid
    #17547
    Depositor
    Domenico Accili
    Insert
    Foxo1 AAA (Foxo1 Mouse)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Changed T24A T253A S316A
    Promoter
    CMV
    Available since
    Oct. 20, 2008
    Availability
    Academic Institutions and Nonprofits only

  26. Lazarus Gata 2 flag

    Plasmid
    #80137
    Purpose
    retroviral expression of Gata2-Flag
    Depositor
    Ellen Rothenberg
    Insert
    Gata2 (Gata2 Mouse)
    Use
    Retroviral
    Tags
    FLAG and IRES-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    80137-plasmid-map-sequence-id-157353

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  28. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

    Plasmid
    #202036
    Purpose
    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato
    Depositor
    Yoav Ben-Simon, Peter Jonas
    Insert
    hM3Dq
    Use
    AAV
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    202036-plasmid-map-sequence-id-405528

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  29. pLV Erh-V5

    Plasmid
    #175123
    Purpose
    Lentiviral expression of mouse Erh-V5
    Depositor
    Larry Gerace
    Insert
    Erh (Erh Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175123-plasmid-map-sequence-id-351935

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  30. pTRIPZ (M)-HT-Cbx2

    Plasmid
    #82510
    Purpose
    Expresses Halotag-Cbx2 fusion proteins in mammalian cells
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 2 (CBX2 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82510-plasmid-map-sequence-id-156621

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  31. pLNCX1 HA AKT2

    Plasmid
    #60129
    Purpose
    expresses HA tagged AKT2
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B (AKT2 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    60129-plasmid-map-sequence-id-96079

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  32. R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid
    #180152
    Purpose
    tdTomato cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180152-plasmid-map-sequence-id-355317

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  33. pAAV-CMV-FLEX-SaCas9-U6-sgNts

    Plasmid
    #159909
    Purpose
    Mutagenesis of Nts
    Depositor
    Larry Zweifel
    Insert
    Nts (Nts Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159909-plasmid-map-sequence-id-317315

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  34. YIp204-PADH1-atTIR1-9myc

    Plasmid
    #99532
    Purpose
    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
    Depositor
    Helle Ulrich
    Insert
    TIR1 (TIR1 Mustard Weed)
    Use
    Tags
    9xMyc
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99532-plasmid-map-sequence-id-192011

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  35. pcDNA3.3_SARS2_XBB.1.16

    Plasmid
    #201189
    Purpose
    expressing SARS-CoV-2 XBB.1.16 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.16) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E180V, T(K)478R on the basis of XBB.1.5
    Promoter
    CMV
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201189-plasmid-map-sequence-id-393640

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  36. pUAST_mLExA-VP16-NFATc1 (C-del)

    Plasmid
    #35023
    Depositor
    Jing-W Wang
    Insert
    NFATc1 (NFATC1 Human)
    Use
    Tags
    VP16 and mLexA
    Expression
    Insect
    Mutation
    deletion of amino acids 590-716
    Promoter
    UAS
    Available since
    March 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    35023-plasmid-map-sequence-id-183825

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  37. pcDNA3.1/HisA/Serca1

    Plasmid
    #74402
    Purpose
    expression of SERCA in mammalian cells
    Depositor
    Robert Nakamoto
    Insert
    SERCA1 (ATP2A1 rabbit)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    NcoI site destroyed (silent) T898
    Promoter
    CMV
    Available since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  38. pAP1-3

    Plasmid
    #71258
    Purpose
    luciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Insert
    3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    GM-CSF minimal
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71258-plasmid-map-sequence-id-131888

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  39. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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  40. pCDNA5 FRT TO FLAG LRRK1

    Plasmid
    #232062
    Purpose
    Expresses FLAG-LRRK1 in mammalian cells
    Depositor
    Samara Reck-Peterson
    Insert
    LRRK1 (LRRK1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232062-plasmid-map-sequence-id-461137

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Showing: 4121 - 4160 of 8321 results