...to breathe!)   Public speaking as a transferable skill The worst thing about doing public speaking as a...Public speaking is not just one of the most common phobias–it’s a key part of being a scientist and an...turned science communicator, I’ve done a lot of public speaking, and I'm happy to share what I've learned...split-second decision, I had always thought I had an obligation to the audience to keep going no matter what,...would let me accomplish my goal.   As a scientist, public speaking isn't a performance art. You’re (usually...goal. Yay!   You’re only human When it comes to public speaking, Murphy’s law definitely applies: what...technical difficulties, and runs into unexpected stumbling blocks. The problem is, when it happens to other...

...world of science publishing, and an ever increasing number of journals and publishers to choose from, ...the manuscript is accepted for publication and is, in most cases, blind (meaning you don’t know who the... to be revised before it can be published, this can delay publication and manuscripts may go through several...should I choose? Writing a research paper for publication in a scientific journal requires several considerations...considerations. First, it is important to establish the type of audience you want to reach with your paper...research may stipulate that your article must be published in an open access journal or by using the open...open access option in a conventional journal. Publishing via open access means that your manuscript will...

...open-access journal that enables high school students to publish peer-reviewed scientific research. You’ll meet ...Arooba Ahmed, Jiachen Lee, and Jillian Parker who published their work on a protein required for cytokinesis...

...onto data longer before publishing. But there are strong incentives to publish such as precedence, recognition...are also taking root. Nature Publishing Group recently launched a publication called Scientific Data, which...This post was contributed by Jim Woodgett. Public Library of Science (PLOS) created a stir earlier this...sharing policy. Since early March, the open access publisher has required authors to include a note as to where...data supporting the research reported in PLOS publications. The policy was not an overnight revelation,...result of consultations between researchers and publishers. Nonetheless, the initial release caused a storm... is founded on the principle that scientific publications must contain enough information (or links to...

...gene of poorly classified function and a well established cellular signaling gene, thus providing a starting...historical standard. Specifically, in our recent publications, we used the cancer plasmid collections listed...pathway reference collection is described in this publication We also wrote an extensive review on the exciting...data generated from these alleles in the above publications), it will be possible for many others to perform...

... : Kwabena's public health research, the HEAD outreach program, and educating the public about dietary... public health...

...Initiative. Endy's team made news in March with a publication in Science describing the development of “transcriptors...are now available at Addgene through the Biobrick Public Agreement (BPA) Plasmid Collection along with a...It's wonderful to have Addgene as a professional public-benefit partner who shares our core values." Addgene...BIOFAB and BIL gates plasmid kits, the Biobricks Public Agreement, and the future of the bioeconomy. Addgene...

...Cas14 to the mix, you get the ultimate combination enabling the detection of RNA, dsDNA, and now ssDNA. By...

...infection and selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence...124(6):1283-98 (PubMed PMID 16564017 ) in all publications arising from the use of this vector. A.2 Map...Biosafety Guide . Back to Top H. References H.1. Published Articles Functional siRNAs and miRNAs exhibit ...

... bacteria for a longer time, you will need to establish glycerol stocks. The addition of glycerol stabilizes...

...above result represents a single experiment. For publishable data, the experiment would need to be repeated...

...observed a sharp decrease in success rate when assembling more than five fragments at a time. Resources...

...Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua C., Vivian...

...deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted to alternative...

...quantification. This protocol was modified from the publication Lock et al. (2014) (Link opens in a new window...

... sample. This protocol was modified from the publication Wang et al. (2018) . Before Starting Thaw the...

...researchers to share data thereby enabling you to do modified analyses on published data if you need to. Similarly...previously published results. This over-reliance on the perfect story for a “high impact” publication can lead...that only these positive results will be published. The publication record will then show that the tested...like ReFigure which aims to consolidate published and unpublished experiments on similar subjects in a single...are new publishing outlets like F1000, PeerJ, PLoS, and bioRxiv that make it easier to publish (or pre-publish...that promote reproducible publishing practices. One example is the publication of peer reviews so we can...speakers from academia, industry, nonprofits, and publishing. The livestream video from the event can be found...

... newly published papers that contain that term. You can also set up alerts for new publications from a...topic or that were published by the lab down the hall.  Scientific journals: They publish papers, so naturally...show all publications by an author, total number of citations, and the number of publications that heavily...read the abstract or full text of the paper, publication date, a relevance score, and type of article....of the number of papers with your search term published each year, which is a nice way to see growth or... work, so it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 ...organizations, and universities often tweet about publications in the fields of research they support. Monitoring...

...online form you can submit "Published plasmids" or "Unpublished or pre-publication plasmids" and set a desired...plasmids. You can submit "Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that...plasmid(s) online (left), choose to submit published or unpublished plasmid(s). Fields marked with an asterisk...in PubMed and choose the appropriate publication. This publication will be listed at the top of the final...that will not be published are accepted. Addgene also encourages submitting pre-publication plasmids so they...are happy to update the publication information once the manuscript is published in a peer-reviewed journal...desired distribution status. You can submit "Published plasmids": This option allows you to quickly associate...

...proceeding in 99% of 12-fragment assemblies and over 90% for 24-fragment assemblies (Figure 3, Table 1). These...for Golden Gate Assemblies with BsaI-HFv2 and T4 DNA ligase. Efficiency of assemblies per plate using ...reaction to form complex, multi-insert modular assemblies that enable biosynthetic pathway engineering ...optimization. However, current best practices for assemblies of more than 10 modules often rely on two-step...placed practical limits on single- or two-step assemblies. We have reduced these limitations through the...that it is now possible to achieve 20+ fragment assemblies with both robust efficiency and accuracy. Golden...cassette. Additional confirmation of accurate assemblies was achieved by the sequencing of plasmids isolated...