We narrowed to 4,495 results for: Ado

1. pT3TS-Hf-RfxCas13d-HA

   Plasmid

   #234479

   Purpose

   Plasmid to carry out IVT of High-fidelity RfxCas13d (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mutation

   Changed four alanine to valine in positions 134, …

   Promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-mTubb3

   Plasmid

   #87116

   Purpose

   AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro

   Plasmid

   #231973

   Purpose

   Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-KIF1B beta-600-1400

   Plasmid

   #79109

   Purpose

   Mammalian expression of GFP-KIF1B beta-600-1400

   Depositor

   Susanne Schlisio

   Article

   Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

   Insert

   GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   exon 25 missing (natural splice varient)

   Promoter

   CMV

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCRISPaint-mNeon-PuroR [M1G]

   Plasmid

   #171800

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJ208

   Plasmid

   #162681

   Purpose

   Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Replacement of W185-F191 with G251-T472 from MHET…

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-1BPNLS-Cas9-1BPNLS

   Plasmid

   #87108

   Purpose

   BPNLS-Cas9-BPNLS expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGFP-mHP1beta

   Plasmid

   #181901

   Purpose

   Fluorescently tagged HP1beta

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1beta (Cbx1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX-PGK-LAMP1-EYFP

   Plasmid

   #164215

   Purpose

   pLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   PGK

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 NL-BCL2L1

    Plasmid

    #113458

    Purpose

    NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 like 1 (BCL2L1 Human)
    

    Use

    Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCJ191

    Plasmid

    #162668

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPaint-mNeon-BlastR [M1G]

    Plasmid

    #171805

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLXI_TRC403 LacZ

    Plasmid

    #111184

    Purpose

    Control inducible vector with LacZ (V5 tagged)

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    lacZ
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. iChr2-Control-Mosaic (SO274)

    Plasmid

    #99751

    Purpose

    Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    HIs-H2B-Cherry, H2B-EGFP, HA-H2B-Cerulean
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLXI_TRC401 LacZ

    Plasmid

    #111183

    Purpose

    Control inducible vector with LacZ (V5 tagged)

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    lacZ
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

    Plasmid

    #156402

    Purpose

    Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRosa26-4xInsulator (JP6)

    Plasmid

    #99627

    Purpose

    IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ai14-HITI

    Plasmid

    #87117

    Purpose

    AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. VARP GFP-pLXIN

    Plasmid

    #62950

    Purpose

    full length human VARP with C-terminal EGFP tag cloned into pLXIN

    Depositor

    Paul Luzio

    Article

    Hesketh et al Dev Cell. 2014 Jun 9;29(5):591-606. doi:

    Insert

    VARP (ANKRD27 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations of amino acids 422-428 to produc…

    Promoter

    LTR

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLZRS-ER-Rac1-Q61L-shortlinker

    Plasmid

    #128584

    Purpose

    For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    Modified hormone binding domain of ER alpha

    Expression

    Mammalian

    Mutation

    Changed Glutamine61 to Leucine in the RAC1 moiety.

    Promoter

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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