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Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorInsertM(BRCT)TdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CBP-HAT-pET
Plasmid#21091DepositorInsertCBP (Crebbp Mouse)
UseTagsHis and T7 tagExpressionBacterialMutationHAT domain aa 1171-1621PromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-cpLuc
Plasmid#135628PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)DepositorInsertNAD-cpLuc sensor
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG-F6C
Plasmid#176546PurposeExpresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
UseTagsHis tagExpressionBacterialMutationchange phenylalanine 6 to cysteine; change glutam…PromoterT5Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-Prp18
Plasmid#111354Purposeprotein purificationDepositorInsertPRP18
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6_rpoA-C
Plasmid#190966PurposeTest the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
UseTagsExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo3
Plasmid#25103DepositorInsertpro-SUMO3 (SUMO3 Human)
UseTagsHisExpressionBacterialMutationchanged Serine 2 to AlaninePromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
5787 pET28aSX104B/ pES42
Plasmid#1229DepositorInsertHsp104 (HSP104 Budding Yeast)
UseTagsHisExpressionBacterialMutationnonePromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFS_0143-pET30-Fusicatenibacter_saccharivorans
Plasmid#116952PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-ChE1 v4.5
Plasmid#185755PurposeBacterial expression of ChE1 v4.5DepositorInsertChE1 v4.5
UseTagsHis10ExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET14b PHAS-I F113A
Plasmid#15680DepositorInsertPHAS-I F113A (Eif4ebp1 Rat)
UseTags6xHisExpressionBacterialMutationF113A mutation.PromoterAvailable sinceSept. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmBelle_B
Plasmid#145963PurposeBacterial Expression of DmBelleDepositorInsertDmBelle (bel Fly)
UseTagsExpressionBacterialMutationdel 155-157, T530A and I685VPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hisx6-Sirt6
Plasmid#20277DepositorInsertSirt6 (Sirt6 Mouse)
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-Luc
Plasmid#135627PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)DepositorInsertNAD-Luc sensor
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30-hDlg PDZ2
Plasmid#21692DepositorInserthDlg/SAP97 PDZ2 domain (DLG1 Human)
UseTagsHis6ExpressionBacterialMutationhuman hDlg/SAP97 PDZ2 domain (residues 318-405)PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del4 pET28C
Plasmid#122118PurposeExpression Brg1 amino acids 1446-1647 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del3 pET28a
Plasmid#122117PurposeExpression Brg1 amino acids 836-1446 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb719-HO
Plasmid#141200PurposeExpresses mRhubarb719 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb719
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, Y198S, T202Y, V203I, W309R, Q310A compared…PromoterT7Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only