We narrowed to 14,579 results for: pET

1. pET19-M(BRCT)TdTwt

   Plasmid

   #207459

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   M(BRCT)TdTwt (Dntt Mouse)
   

   Use

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CBP-HAT-pET

   Plasmid

   #21091

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

   Insert

   CBP (Crebbp Mouse)
   

   Use

   Tags

   His and T7 tag

   Expression

   Bacterial

   Mutation

   HAT domain aa 1171-1621

   Promoter

   Available since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pET51b-NAD-cpLuc

   Plasmid

   #135628

   Purpose

   Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)

   Depositor

   Kai Johnsson

   Article

   Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

   Insert

   NAD-cpLuc sensor
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-T5-FB-E25TAG-F6C

   Plasmid

   #176546

   Purpose

   Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

   Depositor

   Han Xiao

   Article

   Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

   Insert

   IgG affinity protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   change phenylalanine 6 to cysteine; change glutam…

   Promoter

   T5

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-DUET-Prp18

   Plasmid

   #111354

   Purpose

   protein purification

   Depositor

   Christine Guthrie

   Insert

   PRP18
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-PLS-KP6_rpoA-C

   Plasmid

   #190966

   Purpose

   Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-baPrs-hdNadV

   Plasmid

   #91950

   Purpose

   pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

   Inserts

   Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
   Phosphoribosyl Pyrophosphate Synthetases
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Leucine 135 to Isoleucine

   Promoter

   T7

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-pro-sumo3

   Plasmid

   #25103

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO3 (SUMO3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Serine 2 to Alanine

   Promoter

   Available since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

9. 5787 pET28aSX104B/ pES42

   Plasmid

   #1229

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   Hsp104 (HSP104 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pFS_0143-pET30-Fusicatenibacter_saccharivorans

    Plasmid

    #116952

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-LaM-6

    Plasmid

    #172771

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-6
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-ChE1 v4.5

    Plasmid

    #185755

    Purpose

    Bacterial expression of ChE1 v4.5

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    ChE1 v4.5
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET14b PHAS-I F113A

    Plasmid

    #15680

    Depositor

    John Lawrence

    Article

    Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

    Insert

    PHAS-I F113A (Eif4ebp1 Rat)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F113A mutation.

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pETM-60-NusA-DmBelle_B

    Plasmid

    #145963

    Purpose

    Bacterial Expression of DmBelle

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    del 155-157, T530A and I685V

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pET51b-NAD-Luc

    Plasmid

    #135627

    Purpose

    Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)

    Depositor

    Kai Johnsson

    Article

    Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

    Insert

    NAD-Luc sensor
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET30-hDlg PDZ2

    Plasmid

    #21692

    Depositor

    James Baleja

    Article

    Liu et al Biochemistry. 2007 Sep 25. 46(38):10864-

    Insert

    hDlg/SAP97 PDZ2 domain (DLG1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    human hDlg/SAP97 PDZ2 domain (residues 318-405)

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

18. Brg1 Del4 pET28C

    Plasmid

    #122118

    Purpose

    Expression Brg1 amino acids 1446-1647 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Brg1 Del3 pET28a

    Plasmid

    #122117

    Purpose

    Expression Brg1 amino acids 836-1446 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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